Wed .19 Oct 2019
TR | RU | UK | KK | BE |

Viridans streptococci

viridans streptococci, viridans streptococci urinary tract infection
The viridans streptococci are a large group of commensal streptococcal Gram-positive bacteria species that are either α-hemolytic, producing a green coloration on blood agar plates hence the name "viridans", from Latin "vĭrĭdis", green, or nonhemolytic The pseudo-taxonomic term "Streptococcus viridans" is often used to refer to this group of species, but writers who do not like to use the pseudotaxonomic term which treats a group of species as if they were one species prefer the terms viridans streptococci, viridans group streptococci VGS, or viridans streptococcal species

These species possess no Lancefield antigens In general, pathogenicity is low


  • 1 Identification
  • 2 Pathology
    • 21 Identification
  • 3 References
  • 4 Further reading


Viridans streptococci can be differentiated from Streptococcus pneumoniae using an optochin test, as viridans streptococci are optochin-resistant; they also lack either the polysaccharide-based capsule typical of S pneumoniae or the Lancefield antigens of the pyogenic members of the genus

Viridans streptococciStreptococcus pneumoniae
Solubility in bileInsolubleSoluble
Fermentation of inulinNot a fermenterFermenter with acid production
Sensitivity to optochinNot sensitiveSensitive
Pathogenicity to miceNonpathogenicPathogenic
Quellung testNegativePositive


The organisms are most abundant in the mouth, and one member of the group, S mutans, is the cause of dental caries in most cases and populations S sanguinis is also another potential cause Others may be involved in other mouth or gingival infections as pericoronitis If they are introduced into the bloodstream, they have the potential of causing endocarditis, in particular in individuals with damaged heart valves They are the most common causes of subacute bacterial endocarditis Viridans streptococci are identified in cases of neonatal infections

Viridans streptococci have the unique ability to synthesize dextrans from glucose, which allows them to adhere to fibrin-platelet aggregates at damaged heart valves This mechanism underlies their ability to cause subacute valvular heart disease following their introduction into the bloodstream eg, following dental extraction


Phenotypic and biochemical identification Identification of VGS to the species level can be difficult, and phenotypic identification is not always accurate The name “viridans” is somewhat of a misnomer, as many species do not produce any hemolysis on blood agar From a classification perspective, it is not useful to try to differentiate alpha-hemolysis from a lack of hemolysis on blood agar plates sometimes referred to as “gamma-hemolysis”; this feature can vary widely with the growth medium used to cultivate the organism, as well as the incubation temperature Lack of alpha hemolysis does not seem to correlate with the clinical outcome or severity of disease; no enzymatic or toxigenic effect has ever been documented as a by-product of alpha hemolysis

The VGS are a group of catalase-negative, Gram-positive cocci with a chaining morphology on microscopic examination They are leucine aminopeptidase positive, pyrrolidonylarylamidase negative, and do not grow in 65% NaCl, and almost all species are negative for growth on bile esculin agar They differ from pneumococci in that they are optochin resistant and are not bile soluble However, Richter et al examined misidentification of VGS submitted to antimicrobial surveillance programs as pneumococci and found that the distinction of S pneumoniae from the VGS can be difficult, which is not surprising in light of the fact that S mitis and S oralis possess >99% sequence homology with S pneumoniae 47 The authors also found that optochin disk testing did not perform as well as bile solubility testing for identification; in a survey of 1,733 isolates tested, bile solubility testing had higher sensitivity and specificity for differentiation of VGS from pneumococci 47

S anginosus group The S anginosus group of organisms can be beta-, alpha- or nonhemolytic The isolates lacking beta-hemolysis are generally those grouped with the VGS S constellatus is the most likely of this group to be beta-hemolytic There is some evidence implicating beta-hemolytic S constellatus subsp pharyngis as a cause of pharyngitis 55 S intermedius is the species in this group most commonly isolated from brain and liver abscesses The S anginosus group can possess Lancefield group antigens A, C, G, and F, although S intermedius almost never possesses Lancefield group antigens Isolates of the S anginosus group have a characteristic “butterscotch” odor Members of the group are universally positive for three biochemical reactions:acetoin production from glucose positive Vogues-Proskauer reaction, arginine, and sorbitol These are very useful for the differentiation of this group from other VGS

S mitis group The S mitis group of organisms contains several species and is biochemically very inert, which can make species level identification very challenging The use of invalid species names has also been a particular problem with the S mitis group Isolates in this group are negative for acetoin production, arginine, esculin, and mannitol and are sorbitol fermentation negative 14 S pneumoniae is a recently characterized member of the S mitis group 1 As the organism is closely related to S pneumoniae and other S mitis group organisms, accurate identification can be difficult S pseudopneumoniae lacks the pneumococcal capsule and is resistant to optochin when incubated in an atmosphere with elevated CO2 but is susceptible when incubated in ambient air Bile solubility is a more specific test for S pseudopneumoniae than optochin susceptibility, as the organism is not bile soluble 1

S sanguinis group The genetically heterogeneous S sanguinis group was formerly known as S sanguinis Some taxonomists have lumped the S sanguinis group in with the S mitis group based on 16S rRNA gene sequence analysis, but S sanguinis group organisms exhibit divergent phenotypic characteristics Isolates in the S sanguinis group are arginine and esculin positive Like members of the S mitis group, they are negative for acetoin production and mannitol and sorbitol fermentation The S sanguinis group includes S sanguinis, S parasanguinis, and S gordonii 14

S bovis group The S bovis group includes S equinus, S gallolyticus, S infantarius, and other closely related species; they are the nonenterococcal group D streptococci Members of this group are esculin positive and sorbitol negative and produce acetoin Historically, S bovis had been divided into three biotypes:I mannitol fermentation positive, II/1 mannitol negative and beta-glucuronidase negative, and II/2 mannitol negative and beta-glucuronidase positive 14 Further characterization demonstrated that biotype II/1 was S infantarius subsp coli and II/2 was S gallolyticus subsp pasteruianus Isolates from the S bovis group are most frequently encountered in blood cultures from patients with colon cancer However, S bovis group organisms especially S gallolyticus subsp gallolyticus and S infantarius subsp coli have been associated with endocarditis 3 Although infection with S bovis group organisms occurs with higher frequency in adults than in pediatric patients, these organisms have been reported to cause neonatal sepsis and meningitis 20

S salivarius group The S salivarius group is closely related to the S bovis group Species from this group that have been isolated from human infection include S salivarius and S vestibularis, as well as S thermophilus, which has been identified from dairy products S salivarius group organisms are positive for acetoin production and are esculin positive but are negative for arginine hydrolysis and fermentation of mannitol and sorbitol

S mutans group Members of the S mutans group are primarily isolated from the human oral cavity and includes several species that are phenotypically similar S mutans and S sobrinus are the species within this group most commonly isolated from human infection They do not hydrolyze arginine but are positive for acetoin production, esculin hydrolysis, and mannitol and sorbitol fermentation

Automated biochemical methods for identification For the VGS, the use of automated systems for identification has historically been reported as problematic, and this theme applies to multiple automated methodologies One of the major factors affecting the quality of the identifications generated is that the systems may not have all species represented in their databases 32 In one investigation comparing the accuracy of the Vitek2 ID-GPC card to that of conventional agar-based biochemical methods for identification of a variety of Gram-positive and Gram-negative organisms, 72% of Gram-positive isolates were accurately identified by the Vitek2 system 21 Among the most problematic identifications whether incorrectly identified or unresolved were the VGS; S anginosus, S mutans, and S sanguinis were misidentified as other VGS species and, in some instances, as S pneumoniae 21

An investigation into the ability of the BD Phoenix system SMIC/ID panel to identify Streptococcus spp classified 97 consecutive clinical isolates of streptococci, including 34 isolates of VGS, with biochemical methods as the reference method 26 Ninety-one percent of the streptococcal isolates showed agreement between the Phoenix and the reference method Of the 12 S mitis group isolates tested, the Phoenix system correctly identified 7 isolates, with 2 discordant identifications, and there were 3 isolates for which the Phoenix system did not produce any identification Of 22 S anginosus group isolates, 18 were correctly identified and 4 were discordant A second study evaluated the Phoenix SMIC/ID-2 panel using the API 20 Strep system as a comparator method resolving discrepant results via 16S rRNA gene sequencing and amplification and sequencing of housekeeping genes 5 For the VGS, 31 isolates were assayed, with only 53% concordance between the Phoenix and reference methods for S mitis group organisms, 100% concordance for the S anginosus group, and 75% concordance for S sanguinis group organisms 5

Sequence-based identification Historically, DNA-DNA hybridization studies have been used to confirm species level identifications for the VGS However, these procedures are not practical for clinical laboratories to use for identification of these organisms Other sequence-based identification systems have subsequently been introduced for VGS species level identification In general, 16S rRNA gene sequencing results in poor resolution to species level in the VGS This is due to the high degree of 16S rRNA gene homology in this group of organisms; S mitis, S oralis, S pseudopneumoniae, and S pneumoniae almost always have >99% sequence homology in this gene In light of the high degree of 16S rRNA gene sequence similarity, sequencing of alternative gene targets for reliable identification to the species level has been explored One promising target, rnpB, was explored by Innings et al, who analyzed 2 variable regions of rnpB by pyrosequencing 28 Of the 43 species analyzed, all were identified to species level, except for 2 isolates:S anginosus/S constellatus and S infantis/S peroris rnpB can be used for identification of VGS with high-level resolution One other successful approach is sequence analysis of the manganese-dependent superoxide dismutase gene, described by Poyart et al This technique was used to accurately differentiate over 29 streptococcal species, including 16 VGS species, with clear differentiation of S mitis, S oralis, and S pneumoniae 44, 45 Other techniques that have been used, with various degrees of success, are sequence analysis of the 16S-23S intergenic spacer region, d-alanine-d-alanine ligase gene sequencing, and hyaluronate lyase gene sequencing


  1. ^ Dorland's Illustrated Medical Dictionary, headword "streptococcus", subentry "viridans streptococci"
  2. ^ Ryan KJ, Ray CG, eds 2004 Sherris Medical Microbiology 4th ed McGraw Hill pp 293–4 ISBN 0-8385-8529-9 
  3. ^ Viridans Streptococci at the US National Library of Medicine Medical Subject Headings MeSH
  4. ^ Patterson MJ 1996 "Streptococcus" In Baron S; et al Baron's Medical Microbiology 4th ed Univ of Texas Medical Branch ISBN 0-9631172-1-1 
  5. ^ Baucells, BJ; Mercadal Hally, M; Álvarez Sánchez, AT; Figueras Aloy, J 2015 "Asociaciones de probióticos para la prevención de la enterocolitis necrosante y la reducción de la sepsis tardía y la mortalidad neonatal en recién nacidos pretérmino de menos de 1500g:una revisión sistemática" Anales de Pediatría doi:101016/janpedi201507038 ISSN 1695-4033 

Further reading

Naveen Kumar, Venkatesan; van der Linden, Mark; Menon, Thangam; Patric Nitsche-Schmitz, D May 2014 "Viridans and bovis group streptococci that cause infective endocarditis in two regions with contrasting epidemiology" International Journal of Medical Microbiology 304 3-4:262–268 doi:101016/jijmm201310004 Retrieved 31 October 2014 

  • Biology portal

viridans streptococci, viridans streptococci dental, viridans streptococci gram stain, viridans streptococci group, viridans streptococci icd 10, viridans streptococci infection, viridans streptococci morphology, viridans streptococci treatment, viridans streptococci urinary tract infection, viridans streptococci uti

Viridans streptococci Information about

Viridans streptococci

  • user icon

    Viridans streptococci beatiful post thanks!


Viridans streptococci
Viridans streptococci
Viridans streptococci viewing the topic.
Viridans streptococci what, Viridans streptococci who, Viridans streptococci explanation

There are excerpts from wikipedia on this article and video

Random Posts

Body politic

Body politic

The body politic is a metaphor that regards a nation as a corporate entity,2 likened to a human body...


Kakamega is a town in western Kenya lying about 30 km north of the Equator It is the headquarte...
Academic year

Academic year

An academic year is a period of time which schools, colleges and universities use to measure a quant...
Lucrezia Borgia

Lucrezia Borgia

Lucrezia Borgia Italian pronunciation: luˈkrɛttsja ˈbɔrdʒa; Valencian:Lucrècia Borja luˈkrɛsia...