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Transcytosis

transcytosis, transcytosis animation
Transcytosis is a type of transcellular transport in which various macromolecules are transported across the interior of a cell Macromolecules are captured in vesicles on one side of the cell, drawn across the cell, and ejected on the other side Examples of macromolecules transported include IgA, transferrin, and insulin While transcytosis is most commonly observed in cells of an epithelium, the process is also present elsewhere Blood capillaries are a well-known site for transcytosis, though it occurs in other cells, including neurons, osteoclasts and M cells of the intestine

Contents

  • 1 Regulation
  • 2 Role in pathogenesis
  • 3 Clinical Applications of Transcytosis
  • 4 References
  • 5 External links

Regulation

The regulation of transcytosis varies greatly due to the many different tissues in which this process is observed Various tissue specific mechanisms of transcytosis have been identified Brefeldin A, a commonly used inhibitor of ER to Golgi apparatus transport, has been shown to inhibit transcytosis in dog kidney cells which provided the first clues as to the nature of transcytosis regulation Transcytosis in dog kidney cells has also been shown be regulated at the apical membrane by Rab17, as well as Rab11a and Rab25 Further work on dog kidney cells has shown that a signaling cascade involving the phosphorylation of EGFR by Yes leading to the activation of Rab11FIP5 by MAPK1 upregulates transcytosis Transcytosis has been shown to be inhibited by the combination of progesterone and estradiol followed by activation mediated by prolactin in the rabbit mammary gland during pregnancy In the thyroid, follicular cell transcytosis is regulated positively by TSH The phosphorylation of caveolin 1 induced by hydrogen peroxide has been shown to be critical to the activation of transcytosis in pulmonary vascular tissue It can therefore be concluded that the regulation of transcytosis is a complex process that varies between tissues

Role in pathogenesis

Due to the function of transcytosis as a process that transports macromolecules across cells, it can be a convenient mechanism by which pathogens can invade a tissue Transcytosis has been shown to be critical to the entry of Cronobacter sakazakii across the intestinal epithelium as well as the blood–brain barrier Listeria monocytogenes has been shown to enter the intestinal lumen via transcytosis across goblet cells Shiga toxin secreted by enterohemorrhagic E coli has been shown to be transcytosed into the intestinal lumen From these examples, it can be said that transcytosis is vital to the process of pathogenesis for a variety of infectious agents

Clinical Applications of Transcytosis

Pharmaceutical companies, such as Lundbeck, are currently exploring the use of transcytosis as a mechanism for transporting therapeutic drugs across the human blood–brain barrier BBB Exploiting the body’s own transport mechanism can help to overcome the high selectivity of the BBB which typically blocks the uptake of most therapeutic antibodies into the brain and Central Nervous System CNS The pharmaceutical company Genentech, after having synthesized a therapeutic antibody that effectively inhibited BACE1 enzymatic function, experienced problems transferring adequate, efficient levels of the antibody within the brain BACE1 is the enzyme which processes amyloid precursor proteins into amyloid-β peptides, including the species that aggregate to form amyloid plaques associated with Alzheimer's disease

Molecules are transported across an epithelial or endothelial barrier by one of two routes: 1 a trans-cellular route through the intra-cellular compartment of the cell, or 2 a para-cellular route through the extra-cellular space between adjacent cells The trans-cellular route is also called transcytosis Transcytosis can be receptor-mediated and consists of three steps: 1 receptor-mediated endocytosis of the molecule on one side of the cell, eg the luminal side; 2 movement of the molecule through the intracellular compartment typically within the endosomal system; and 3 exocytosis of the molecule to the extracellular space on the other side of the cell, eg the abluminal side

Transcytosis may be either unidirectional or bidirectional Unidirectional transcytosis may occur selectively in the luminal to abluminal direction, or in the reverse direction, in the abluminal to luminal direction

Transcytosis is prominent in brain microvascular peptide and protein transport, because the brain microvascular endothelium, which forms the blood-brain barrier BBB in vivo, expresses unique, epithelial-like, high resistance tight junctions The brain endothelial tight junctions virtually eliminate the para-cellular pathway of solute transport across the microvascular endothelial wall in brain In contrast, the endothelial barrier in peripheral organs does not express tight junctions, and solute movement through the para-cellular pathway is prominent at the endothelial barrier in organs other than the brain or spinal cord

Receptor-mediated transcytosis, or RMT, across the BBB is a potential pathway for drug delivery to the brain, particularly for biologic drugs such as recombinant proteins The non-transportable drug, or therapeutic protein, is genetically fused to a transporter protein The transporter protein may be an endogenous peptide, or peptidomimetic monoclonal antibody, which undergoes RMT across the BBB via transport on brain endothelial receptors such as the insulin receptor or transferrin receptor The transporter protein acts as a molecular Trojan horse to ferry into brain the therapeutic protein that is genetically fused to the receptor-specific Trojan horse protein

Monoclonal antibody Trojan horses that target the BBB insulin or transferrin receptor have been in drug development for over 10 years at ArmaGen, Inc, a biotechnology company in Los Angeles ArmaGen has developed genetically engineered antibodies against both the insulin and transferrin receptors, and has fused to these antibodies different therapeutic proteins, including lysosomal enzymes, therapeutic antibodies, decoy receptors, and neurotrophins These therapeutic proteins alone do not cross the BBB, but following genetic fusion to the Trojan horse antibody, the therapeutic protein penetrates the BBB at a rate comparable to small molecules In 2015, ArmaGen will be the first to enter human clinical trials with the BBB Trojan horse fusion proteins that delivery protein drugs to the brain via the transcytosis pathway The human diseases initially targeted by ArmaGen are lysosomal storage diseases that adversely affect the brain Inherited diseases create a condition where a specific lysosomal enzyme is not produced, leading to serious brain conditions including mental retardation, behavioral problems, and then dementia Although the missing enzyme can be manufactured by drug companies, the enzyme drug alone does not treat the brain, because the enzyme alone does not cross the BBB ArmaGen has re-engineered the missing lysosomal enzyme as a Trojan horse-enzyme fusion protein that crosses the BBB The first clinical trials of the new Trojan horse fusion protein technology will treat the brain in lysosomal storage disorders, including Mucopolysaccharidosis MPS Type I, also called Hurlers syndrome, and MPS Type II, also called Hunter syndrome

Researchers at Genentech proposed the creation of a bispecific antibody that could bind the BBB membrane, induce receptor-mediated transcytosis, and release itself on the other side into the brain and CNS They utilized a mouse bispecific antibody with two active sites performing different functions One arm had a low-affinity anti-transferrin receptor binding site that induces transcytosis A high-affinity binding site would result in the antibody not being able to release from the BBB membrane after transcytosis This way the amount of transported antibody is based on the concentration of antibody on either side of the barrier The other arm had the previously developed high-affinity anti-BACE1 binding site that would inhibit BACE1 function and prevent amyloid plaque formation Genentech was able to demonstrate in mouse models that the new bispecific antibody was able to reach therapeutic levels in the brain Genentech’s method of disguising and transporting the therapeutic antibody by attaching it to a receptor-mediated transcytosis activator has been referred to as the "Trojan Horse" method

References

  1. ^ Perez, J H; Branch, W J; Smith, L; Mullock, B M; Luzio, J P 1988 "Investigation of endosomal compartments involved in endocytosis and transcytosis of polymeric immunoglobulin a by subcellular fractionation of perfused isolated rat liver" The Biochemical Journal 251 3: 763–770 doi:101042/bj2510763 PMC 1149069  PMID 3415644 
  2. ^ Fishman, J B; Rubin, J B; Handrahan, J V; Connor, J R; Fine, R E 1987 "Receptor-mediated transcytosis of transferrin across the blood-brain barrier" Journal of Neuroscience Research 18 2: 299–304 doi:101002/jnr490180206 PMID 3694713 
  3. ^ Duffy, K R; Pardridge, W M 1987 "Blood-brain barrier transcytosis of insulin in developing rabbits" Brain Research 420 1: 32–38 doi:101016/0006-89938790236-8 PMID 3315116 
  4. ^ Williams, S K; Greener, D A; Solenski, N J 1984 "Endocytosis and exocytosis of protein in capillary endothelium" Journal of Cellular Physiology 120 2: 157–162 doi:101002/jcp1041200208 PMID 6430919 
  5. ^ Fabian, R H 1991 "Retrograde axonal transport and transcytosis of immunoglobulins: Implications for the pathogenesis of autoimmune motor neuron disease" Advances in Neurology 56: 433–444 PMID 1853776 
  6. ^ Salo, J; Lehenkari, P; Mulari, M; Metsikkö, K; Väänänen, H K 1997 "Removal of osteoclast bone resorption products by transcytosis" Science 276 5310: 270–273 doi:101126/science2765310270 PMID 9092479 
  7. ^ Landsverk, T 1987 "The follicle-associated epithelium of the ileal Peyer's patch in ruminants is distinguished by its shedding of 50 nm particles" Immunology and Cell Biology 65 3: 251–261 doi:101038/icb198728 PMID 3623609 
  8. ^ Taub, M E; Shen, W C 1993 "Regulation of pathways within cultured epithelial cells for the transcytosis of a basal membrane-bound peroxidase-polylysine conjugate" Journal of Cell Science 106 4: 1313–1321 PMID 8126110 
  9. ^ Hunziker, W; Peters, P J 1998 "Rab17 localizes to recycling endosomes and regulates receptor-mediated transcytosis in epithelial cells" The Journal of Biological Chemistry 273 25: 15734–15741 doi:101074/jbc2732515734 PMID 9624171 
  10. ^ Casanova, J E; Wang, X; Kumar, R; Bhartur, S G; Navarre, J; Woodrum, J E; Altschuler, Y; Ray, G S; Goldenring, J R 1999 "Association of Rab25 and Rab11a with the Apical Recycling System of Polarized Madin–Darby Canine Kidney Cells" Molecular Biology of the Cell 10 1: 47–61 doi:101091/mbc10147 PMC 25153  PMID 9880326 
  11. ^ Su, T; Bryant, D M; Luton, F D R; Vergés, M; Ulrich, S M; Hansen, K C; Datta, A; Eastburn, D J; Burlingame, A L; Shokat, K M; Mostov, K E 2010 "A kinase cascade leading to Rab11-FIP5 controls transcytosis of the polymeric immunoglobulin receptor" Nature Cell Biology 12 12: 1143–1153 doi:101038/ncb2118 PMC 3072784  PMID 21037565 
  12. ^ Rosato, R; Jammes, H; Belair, L; Puissant, C; Kraehenbuhl, J P; Djiane, J 1995 "Polymeric-Ig receptor gene expression in rabbit mammary gland during pregnancy and lactation: Evolution and hormonal regulation" Molecular and Cellular Endocrinology 110 1–2: 81–87 doi:101016/0303-72079503519-d PMID 7672455 
  13. ^ Sun, Y; Hu, G; Zhang, X; Minshall, R D 2009 "Phosphorylation of caveolin-1 regulates oxidant-induced pulmonary vascular permeability via paracellular and transcellular pathways" Circulation Research 105 7: 676–685, 15 685 following 685 doi:101161/CIRCRESAHA109201673 PMC 2776728  PMID 19713536 
  14. ^ Giri, C P; Shima, K; Tall, B D; Curtis, S; Sathyamoorthy, V; Hanisch, B; Kim, K S; Kopecko, D J 2011 "Cronobacter spp previously Enterobacter sakazakii invade and translocate across both cultured human intestinal epithelial cells and human brain microvascular endothelial cells" Microbial Pathogenesis 52 2: 140–7 doi:101016/jmicpath201110003 PMID 22023990 
  15. ^ Nikitas, G; Deschamps, C; Disson, O; Niault, T; Cossart, P; Lecuit, M 2011 "Transcytosis of Listeria monocytogenes across the intestinal barrier upon specific targeting of goblet cell accessible E-cadherin" Journal of Experimental Medicine 208 11: 2263–2277 doi:101084/jem20110560 PMC 3201198  PMID 21967767 
  16. ^ Lukyanenko, V; Malyukova, I; Hubbard, A; Delannoy, M; Boedeker, E; Zhu, C; Cebotaru, L; Kovbasnjuk, O 2011 "Enterohemorrhagic Escherichia coli infection stimulates Shiga toxin 1 macropinocytosis and transcytosis across intestinal epithelial cells" AJP: Cell Physiology 301 5: C1140–C1149 doi:101152/ajpcell000362011 PMC 3213915  PMID 21832249 
  17. ^ Simionescu, N; Simionescu, M 1985 "Interactions of endogenous lipoproteins with capillary endothelium in spontaneously hyperlipoproteinemic rats" Microvascular Research 30 3: 314–332 doi:101016/0026-28628590063-9 PMID 4088094 
  18. ^ Pardridge, WM 1986 "Receptor-mediated peptide transport through the blood-brain barrier" Endocrine Reviews 7 3: 314–330 doi:101210/edrv-7-3-314 PMID 3017689 
  19. ^ Brightman, MW; Reese, TS 1969 "Junctions between intimately apposed cell membranes in the vertebrate brain" Journal of Cell Biology 40 3: 648–677 doi:101083/jcb403648 PMC 2107650  PMID 5765759 
  20. ^ Bundgaard, M 1984 "The three-dimensional organization of tight junctions in a capillary endothelium revealed by serial-section electron microscopy" Journal of Ultrastructural Research 88 1: 1–17 doi:101016/s0022-53208490177-1 PMID 6545375 
  21. ^ Pardridge, WM 2007 "Drug targeting to the brain" Pharmaceutical Research 24 9: 1733–1744 doi:101007/s11095-007-9324-2 PMID 17554607 
  22. ^ Pardridge, WM; Boado, RJ 2012 "Reengineering biopharmaceuticals for targeted delivery across the blood-brain barrier" Methods in Enzymology 503: 269–292 doi:101016/B978-0-12-396962-000011-2 PMID 22230573 
  23. ^ Yu, Y Joy; et al 2001 "Boosting Brain Uptake of a Therapeutic Antibody by Reducing Its Affinity for a Transcytosis Target" Science Translational Medicine 3: 84 doi:101126/scitranslmed3002230 PMID 21613623 

External links

  • Macromolecules Can Be Transferred Across Epithelial Cell Sheets by Transcytosis
  • Transcytosis of IgA
  • Transcytosis of bacteria

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Transcytosis


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    29.10.2014


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