Primary and secondary antibodiesprimary and secondary antibodies, western blot primary and secondary antibodies
Primary and secondary antibodies are two groups of antibodies that are classified based on whether they bind to antigens or proteins directly or target another primary antibody that, in turn, is bound to an antigen or protein
- 1 Primary
- 2 Secondary
- 21 Applications
- 3 References
A primary antibody can be very useful for the detection of biomarkers for diseases such as cancer, diabetes, Parkinson’s and Alzheimer’s disease and they are used for the study of ADME and multi-drug resistance MDR of therapeutic agentsThe primary antibody binds to an antigen in red A labeled secondary antibody in green, then binds to the primary antibody The label is then used to indirectly detect the antigen
Secondary antibody is especially efficient in immunolabeling Secondary antibodies bind to primary antibodies, which are directly bound to the target antigens In immunolabeling, the primary antibody's Fab domain binds to an antigen and exposes its Fc domain to secondary antibody Then, the secondary antibody's Fab domain binds to the primary antibody's Fc domain Since the Fc domain is constant within the same animal class, only one type of secondary antibody is required to bind to many types of primary antibodies This reduces the cost by labeling only one type of secondary antibody, rather than labeling various types of primary antibodies Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody
Secondary antibodies can be conjugated to enzymes such as horseradish peroxidase HRP or alkaline phosphatase AP; or fluorescent dyes such as fluorescein isothiocyanate FITC, rhodamine derivatives, Alexa Fluor dyes; or other molecules to be used in various applications Secondary antibodies are used in many biochemical assays including:
- ELISA, including many HIV tests
- Western blot
- ^ "Secondary Antibodies as Probes" wwwthermofishercom Retrieved 2017-05-31
- ^ "Secondary antibody review based on formal publications" secondary-antibodycom Retrieved 2008-04-16
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Primary and secondary antibodies
Primary and secondary antibodies
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