Peptidyl transferasepeptidyl transferase, peptidyl transferase center
The peptidyl transferase is an aminoacyltransferase EC 23212 as well as the primary enzymatic function of the ribosome, which forms peptide bonds between adjacent amino acids using tRNAs during the translation process of protein biosynthesis The substrates for the peptidyl transferase reaction are two RNA molecules, one bearing the growing peptide chain and the other bearing the amino acid that will be added to the chain The peptidyl chain and the amino acids are attached to their respective tRNAs via ester bonds to the O atom at the CCA-3' ends of these tRNAs
Peptidyl transferase activity is carried out by the ribosome Peptidyl transferase activity is not mediated by any ribosomal proteins but by ribosomal RNA rRNA, a ribozyme Ribozymes are the only enzymes which are not made up of proteins, but ribonucleotides All other enzymes are made up of proteins This RNA relic is the most significant piece of evidence supporting the RNA World hypothesis
- In Prokaryotes, the 50S 23S component ribosome subunit contains the peptidyl transferase component and acts as a ribozyme The peptidyl transferase center on the 50S subunit lies at the lower tips acceptor ends of the A- and O- site tRNAs
- In Eukaryotes, the 60S 28S component ribosome subunit contains the peptidyl transferase component and acts as the ribozyme
Peptidyl transferases are not limited to translation, but there are relatively few enzymes with this function
- 1 Antibiotic target
- 2 See also
- 3 References
- 4 External links
The following protein synthesis inhibitors target peptidyl transferase:
- Chloramphenicol binds to A2451 and A2452 residues in the 23S rRNA of the ribosome and inhibits peptide bond formation
- Pleuromutilins also bind to peptidyl transferase
- Macrolide antibiotics are thought to inhibit peptidyl transferase, in addition to inhibiting ribosomal translocation
- ^ Garret, Grisham, Reginald, Charles Biochemistry 5th Edition pp 437–438
- ^ Garrett, Grisham, Reginald, Charles Biochemistry 5th Edition p 1062
- ^ Gu Z, Harrod R, Rogers EJ, Lovett PS June 1994 "Anti-peptidyl transferase leader peptides of attenuation-regulated chloramphenicol-resistance genes" Proc Natl Acad Sci USA 91 12: 5612–6 doi:101073/pnas91125612 PMC 44046 PMID 7515506
- ^ Long, Katherine S; Hansen, LH; Jakobsen, L; Vester, B April 2006 "Interaction of Pleuromutilin Derivatives with the Ribosomal Peptidyl Transferase Center" PDF Antimicrobial Agents and Chemotherapy 50 4: 1458–1462 doi:101128/AAC5041458-14622006 PMC 1426994 PMID 16569865
- ^ Protein synthesis inhibitors: macrolides mechanism of action animation Classification of agents Pharmamotion Author: Gary Kaiser The Community College of Baltimore County Retrieved on July 31, 2009
- Peptidyl transferases at the US National Library of Medicine Medical Subject Headings MeSH
|231: other than amino-acyl groups||
|233: converted into alkyl on transfer||
- Molecular and Cellular Biology portal
|This EC 23 enzyme-related article is a stub You can help Wikipedia by expanding it
peptidyl transferase, peptidyl transferase action, peptidyl transferase center, peptidyl transferase diagram, peptidyl transferase in prokaryotes, peptidyl transferase location, peptidyl transferase mechanism, peptidyl transferase peptide bond formation, peptidyl transferase region, peptidyl transferase ribozyme
Peptidyl transferase Information about
Peptidyl transferase viewing the topic.
There are excerpts from wikipedia on this article and video
Our site has a system which serves search engine function.
You can search all data in our system with above button which written "What did you look for? "
Welcome to our simple, stylish and fast search engine system.
We have prepared this method why you can reach most accurate and most up to date knowladge. The search engine that developed for you transmits you to the latest and exact information with its basic and quick system.
You can find nearly everything data which found from internet with this system.