Wed . 20 Jul 2020
TR | RU | UK | KK | BE |

Nondisjunction

non disjunction, non disjunction of chromosomes
Nondisjunction is the failure of homologous chromosomes or sister chromatids to separate properly during cell division There are three forms of nondisjunction: failure of a pair of homologous chromosomes to separate in meiosis I, failure of sister chromatids to separate during meiosis II, and failure of sister chromatids to separate during mitosis Nondisjunction results in daughter cells with abnormal chromosome numbers aneuploidy

Calvin Bridges and Thomas Hunt Morgan are credited with discovering nondisjunction in Drosophila melanogaster sex chromosomes in the spring of 1910, while working in the Zoological Laboratory of Columbia University

1 Meiosis I 2 Meiosis II 3 Fertilization 4 Zygote The left image at the blue arrow is nondisjunction taking place during meiosis II The right image at the green arrow is nondisjunction taking place during meiosis I Nondisjunction is when chromosomes fail to separate normally resulting in a gain or loss of chromosomes

Contents

  • 1 Types
    • 11 Meiosis II
    • 12 Mitosis
  • 2 Molecular mechanisms
    • 21 Central role of the spindle assembly checkpoint
    • 22 Sex-specific differences in meiosis
    • 23 Age-related loss of cohesin ties
  • 3 Consequences
    • 31 Monosomy
      • 311 Turner syndrome X monosomy 45, X0
    • 32 Autosomal trisomy
      • 321 Down syndrome trisomy 21
      • 322 Edwards syndrome trisomy 18 and Patau syndrome trisomy 13
    • 33 Sex chromosome aneuploidy
      • 331 Klinefelter syndrome 47, XXY
      • 332 XYY Male 47, XYY
      • 333 Trisomy X 47,XXX
    • 34 Uniparental disomy
    • 35 Mosaicism syndromes
    • 36 Mosaicism in malignant transformation
  • 4 Diagnosis
    • 41 Preimplantation genetic diagnosis
    • 42 Karyotyping
    • 43 Polar body diagnosis
    • 44 Blastomere biopsy
  • 5 Lifestyle/environmental hazards
  • 6 References

Types

In general, nondisjunction can occur in any form of cell division that involves ordered distribution of chromosomal material Higher animals have three distinct forms of such cell divisions: Meiosis I and meiosis II are specialized forms of cell division occurring during generation of gametes eggs and sperm for sexual reproduction, mitosis is the form of cell division used by all other cells of the body

Meiosis II

Ovulated eggs become arrested in metaphase II until fertilization triggers the second meiotic division Similar to the segregation events of mitosis, the pairs of sister chromatids resulting from the separation of bivalents in meiosis I are further separated in anaphase of meiosis II In oocytes, one sister chromatid is segregated into the second polar body, while the other stays inside the egg During spermatogenesis, each meiotic division is symmetric such that each primary spermatocyte gives rise to 2 secondary spermatocytes after meiosis I, and eventually 4 spermatids after meiosis II
Meiosis II-nondisjunction may also result in aneuploidy syndromes, but only to a much smaller extent than do segregation failures in meiosis I

Nondisjunction of sister chromatids during mitosis:
Left: Metaphase of mitosis Chromosome line up in the middle plane, the mitotic spindle forms and the kinetochores of sister chromatids attach to the microtubules
Right: Anaphase of mitosis, where sister chromatids separate and the microtubules pull them in opposite directions
The chromosome shown in red fails to separate properly, its sister chromatids stick together and get pulled to the same side, resulting in mitotic nondisjunction of this chromosome

Mitosis

Division of somatic cells through mitosis is preceded by replication of the genetic material in S phase As a result, each chromosome consists of two sister chromatids held together at the centromere In the anaphase of mitosis, sister chromatids separate and migrate to opposite cell poles before the cell divides Nondisjunction during mitosis leads to one daughter receiving both sister chromatids of the affected chromosome while the other gets none This is known as a chromatin bridge or an anaphase bridge Mitotic nondisjunction results in somatic mosaicism, since only daughter cells originating from the cell where the nondisjunction event has occurred will have an abnormal number of chromosomes Nondisjunction during mitosis can contribute to the development of some forms of cancer, eg retinoblastoma see below Chromosome nondisjunction in mitosis can be atrributed to the inactivation of topoisomerase II, condensin, or separase Meiotic nondisjunction has been well studied in Saccharomyces cerevisiae This yeast undergoes mitosis similarly to other eukaryotes Chromosome bridges occur when sister chromatids are held together post replication by DNA-DNA topological entanglement and the cohesion complex During anaphase, cohesin is cleaved by separase Topoisomerase II and condensin are responsible for removing catenations

Molecular mechanisms

Central role of the spindle assembly checkpoint

The spindle assembly checkpoint SAC is a molecular safe-guarding mechanism that governs proper chromosome segregation in eukaryotic cells SAC inhibits progression into anaphase until all homologous chromosomes bivalents, or tetrads are properly aligned to the spindle apparatus Only then, SAC releases its inhibition of the anaphase promoting complex APC, which in turn irreversibly triggers progression through anaphase

Sex-specific differences in meiosis

Surveys of cases of human aneuploidy syndromes have shown that most of them are maternally derived This raises the question: Why is female meiosis more error prone The most obvious difference between female oogenesis and male spermatogenesis is the prolonged arrest of oocytes in late stages of prophase I for many years up to several decades Male gametes on the other hand quickly go through all stages of meiosis I and II Another important difference between male and female meiosis concerns the frequency of recombination between homologous chromosomes: In the male, almost all chromosome pairs are joined by at least one crossover, while more than 10% of human oocytes contain at least one bivalent without any crossover event Failures of recombination or inappropriately located crossovers have been well documented as contributors to the occurrence of nondisjunction in humans

Age-related loss of cohesin ties

Due to the prolonged arrest of human oocytes, weakening of cohesive ties holding together chromosomes and reduced activity of the SAC may contribute to maternal age-related errors in segregation control The cohesin complex is responsible for keeping together sister chromatids and provides binding sites for spindle attachment Cohesin is loaded onto newly replicated chromosomes in oogonia during fetal development Mature oocytes have only limited capacity for reloading cohesin after completion of S phase The prolonged arrest of human oocytes prior to completion of meiosis I may therefore result in considerable loss of cohesin over time Loss of cohesin is assumed to contribute to incorrect microtubule-kinetochore attachment and chromosome segregation errors during meiotic divisions

Consequences

The result of this error is a cell with an imbalance of chromosomes Such a cell is said to be aneuploid Loss of a single chromosome 2n-1, in which the daughter cells with the defect will have one chromosome missing from one of its pairs, is referred to as a monosomy Gaining a single chromosome, in which the daughter cells with the defect will have one chromosome in addition to its pairs is referred to as a trisomy In the event that an aneuploidic gamete is fertilized, a number of syndromes might result

Monosomy

The only known survivable monosomy in humans is Turner syndrome, where the affected individual is monosomic for the X chromosome see below Other monosomies are usually lethal during early fetal development, and survival is only possible if not all the cells of the body are affected in case of a mosaicism see below, or if the normal number of chromosomes is restored via duplication of the single monosomic chromosome "chromosome rescue"

Turner syndrome X monosomy 45, X0

Karyotype of X monosomy Turner syndrome
This condition is characterized by the presence of only one X chromosome and no Y chromosome see bottom right corner

Complete loss of an entire X chromosome accounts for about half the cases of Turner syndrome The importance of both X chromosomes during embryonic development is underscored by the observation that the overwhelming majority >99% of fetuses with only one X chromosome karyotype 45, X0 are spontaneously aborted

Autosomal trisomy

The term autosomal trisomy means that a chromosome other than the sex chromosomes X and Y is present in 3 copies instead of the normal number of 2 in diploid cells

Down syndrome trisomy 21

Karyotype of trisomy 21 Down syndrome
Note that chromosome 21 is present in 3 copies, while all other chromosomes show the normal diploid state with 2 copies Most cases of trisomy of chromosome 21 are caused by a nondisjunction event during meiosis I see text

Down syndrome, a trisomy of chromosome 21, is the most common anomaly of chromosome number in humans The majority of cases results from nondisjunction during maternal meiosis I Trisomy occurs in at least 03% of newborns and in nearly 25% of spontaneous abortions It is the leading cause of pregnancy wastage and is the most common known cause of mental retardation It is well documented that advanced maternal age is associated with greater risk of meiotic nondisjunction leading to Down syndrome This may be associated with the prolonged meiotic arrest of human oocytes potentially lasting for more than four decades

Edwards syndrome trisomy 18 and Patau syndrome trisomy 13

Human trisomies compatible with live birth, other than Down syndrome trisomy 21, are Edwards syndrome trisomy 18 and Patau syndrome trisomy 13 Complete trisomies of other chromosomes are usually not viable and represent a relatively frequent cause of miscarriage Only in rare cases of a mosaicism, the presence of a normal cell line, in addition to the trisomic cell line, may support the development of a viable trisomy of the other chromosomes

Sex chromosome aneuploidy

The term sex chromosome aneuploidy summarizes conditions with an abnormal number of sex chromosomes, ie other than XX female or XY male Formally, X chromosome monosomy Turner syndrome, see above can also be classified as a form of sex chromosome aneuploidy

Klinefelter syndrome 47, XXY

Klinefelter syndrome is the most common sex chromosome aneuploidy in humans It represents the most frequent cause of hypogonadism and infertility in men Most cases are caused by nondisjunction errors in paternal meiosis I About eighty percent of individuals with this syndrome have one extra X chromosome resulting in the karyotype XXY The remaining cases have either multiple additional sex chromosomes 48,XXXY; 48,XXYY; 49,XXXXY, mosaicism 46,XY/47,XXY, or structural chromosome abnormalities

XYY Male 47, XYY

The incidence of XYY syndrome is approximately 1 in 800-1000 male births Many cases remain undiagnosed because of their normal appearance and fertility, and the absence of severe symptoms The extra Y chromosome is usually a result of nondisjunction during paternal meiosis II

Trisomy X 47,XXX

Trisomy X is a form of sex chromosome aneuploidy where females have three instead of two X chromosomes Most patients are only mildly affected by neuropsychological and physical symptoms Studies examining the origin of the extra X chromosome observed that about 58-63% of cases were caused by nondisjunction in maternal meiosis I, 16-18% by nondisjunction in maternal meiosis II, and the remaining cases by post-zygotic, ie mitotic, nondisjunction

Uniparental disomy

Uniparental disomy denotes the situation where both chromosomes of a chromosome pair are inherited from the same parent and are therefore identical This phenomenon most likely is the result of a pregnancy that started as a trisomy due to nondisjunction Since most trisomies are lethal, the fetus only survives because it loses one of the three chromosomes and becomes disomic Uniparental disomy of chromosome 15 is, for example, seen in some cases of Prader-Willi syndrome and Angelman syndrome

Mosaicism syndromes

Mosaicism syndromes can be caused by mitotic nondisjunction in early fetal development As a consequence, the organism evolves as a mixture of cell lines with differing ploidy number of chromosomes Mosaicism may be present in some tissues, but not in others Affected individuals may have a patchy or assymmetric appearance Examples of mosaicism syndromes include Pallister-Killian syndrome and Hypomelanosis of Ito

Mosaicism in malignant transformation

Loss of a tumor suppressor gene locus according to the two-hit model:
In the first hit, the tumor suppressor gene on one of the two chromosomes is affected by a mutation that makes the gene product non-functional This mutation may arise spontaneously as a DNA replication error or may be induced by a DNA damaging agent The second hit removes the remaining wild-type chromosome, for example through a mitotic nondisjunction event There are several other potential mechanisms for each of the two steps, for example an additional mutation, an unbalanced translocation, or a gene deletion by recombination As a result of the double lesion, the cell may become malignant because it is no longer able to express the tumor suppressor protein

Development of cancer often involves multiple alterations of the cellular genome Knudson hypothesis Human retinoblastoma is a well studied example of a cancer type where mitotic nondisjunction can contribute to malignant transformation: Mutations of the RB1 gene, which is located on chromosome 13 and encodes the tumor suppressor retinoblastoma protein, can be detected by cytogenetic analysis in many cases of retinoblastoma Mutations of the RB1 locus in one copy of chromosome 13 are sometimes accompanied by loss of the other wild-type chromosome 13 through mitotic nondisjunction By this combination of lesions, affected cells completely lose expression of functioning tumor suppressor protein

Diagnosis

Preimplantation genetic diagnosis

Pre-implantation genetic diagnosis PGD or PIGD is a technique used to identify genetically normal embryos and is useful for couples who have a family history of genetic disorders This is an option for people choosing to procreate through IVF PGD is considered difficult due to it being both time consuming and having success rates only comparable to routine IVF

Karyotyping

Karyotyping involves performing an amniocentesis in order to study the cells of an unborn fetus during metophase 1 Light microscopy can be used to visually determine if aneuploidy is an issue

Polar body diagnosis

Polar body diagnosis PBD can be use to detect maternally derived chromosomal aneuploidies as well as translocations in oocytes The advantage of PBD over PGD is that it can be accomplished in a short amount of time This is accomplished through zona drilling or laser drilling

Blastomere biopsy

Blastomere biopsy is a technique in which blastomeres are removed from the zona pellucida It is commonly used to detect aneuploidy Genetic analysis is conducted once the procedure is complete Additional studies are needed to assess the risk associated with the procedure

Lifestyle/environmental hazards

Exposure of spermatozoa to lifestyle, environmental and/or occupational hazards may increase the risk of aneuploidy Cigarette smoke is a known aneugen aneuploidy inducing agent It is associated with increases in aneuploidy ranging from 15 to 30-fold Other studies indicate factors such as alcohol consumption, occupational exposure to benzene, and exposure to the insecticides fenvalerate and carbaryl also increase aneuploidy

References

  1. ^ a b Simmons, D Peter Snustad, Michael J 2006 Principles of genetics 4 ed New York, NY : Wiley ISBN 9780471699392 
  2. ^ a b c d e f g h i Bacino, CA; Lee, B 2011 "Chapter 76: Cytogenetics" In Kliegman, RM; Stanton, BF; St Geme, JW; Schor, NF; Behrman, RE Nelson Textbook of Pediatrics, 19th Edition 19th ed Philadelphia: Saunders pp 394–413 ISBN 9781437707557 
  3. ^ a b c d Strachan, Tom; Read, Andrew 2011 Human molecular genetics 4th ed New York: Garland Science ISBN 9780815341499 
  4. ^ Thomas Hunt Morgan August 31, 2012 Sex-Linked Inheritance in Drosophila Ulan Press pp 10–11 
  5. ^ a b c Nagaoka, SI; Hassold, TJ; Hunt, PA Jun 18, 2012 "Human aneuploidy: mechanisms and new insights into an age-old problem" Nature Reviews Genetics 13 7: 493–504 doi:101038/nrg3245 PMC 3551553  PMID 22705668 
  6. ^ a b c Jones, K T; Lane, S I R 27 August 2013 "Molecular causes of aneuploidy in mammalian eggs" Development 140 18: 3719–3730 doi:101242/dev090589 PMID 23981655 
  7. ^ a b eds, Charles R Scriver ,; et al 2005 The online metabolic & molecular bases of inherited disease 8th ed New York: McGraw-Hill ISBN 9780079130358 
  8. ^ Quevedo, O; García-Luis, J; Matos-Perdomo, E; Aragón, L; Machín, F 2012 "Nondisjunction of a single chromosome leads to breakage and activation of DNA damage checkpoint in G2" PLOS Genetics 8 2: e1002509 doi:101371/journalpgen1002509 PMC 3280967  PMID 22363215 
  9. ^ Vaahtokari, A; Aberg, T; Thesleff, I Jan 1996 "Apoptosis in the developing tooth: association with an embryonic signaling center and suppression by EGF and FGF-4" Development 122 1: 121–9 PMID 8565823 
  10. ^ Banks, P Feb 1977 "Pulp changes after anterior mandibular subapical osteotomy in a primate model" Journal of maxillofacial surgery 5 1: 39–48 doi:101016/s0301-05037780074-x PMID 0403247 
  11. ^ Holm, C; Goto, T; Wang, JC; Botstein, D Jun 1985 "DNA topoisomerase II is required at the time of mitosis in yeast" Cell 41 2: 553–63 doi:101016/s0092-86748580028-3 PMID 2985283 
  12. ^ Sun, S-C; Kim, N-H 14 November 2011 "Spindle assembly checkpoint and its regulators in meiosis" Human Reproduction Update 18 1: 60–72 doi:101093/humupd/dmr044 PMID 22086113 
  13. ^ a b Eichenlaub-Ritter, Ursula 2012 "Oocyte ageing and its cellular basis" The International Journal of Developmental Biology 56 10–11–12: 841–852 doi:101387/ijdb120141ue 
  14. ^ a b c d Gleason, H William Taeusch, Roberta A Ballard, Christine A 2005 Avery's diseases of the newborn 8th ed Philadelphia, Pa: WB Saunders ISBN 0721693474 CS1 maint: Extra text: authors list link
  15. ^ Koehler, KE; Hawley, RS; Sherman, S; Hassold, T 1996 "Recombination and nondisjunction in humans and flies" Human Molecular Genetics 5 Spec No: 1495–504 doi:101093/hmg/5Supplement_11495 PMID 8875256 
  16. ^ Tartaglia, NR; Howell, S; Sutherland, A; Wilson, R; Wilson, L May 11, 2010 "A review of trisomy X 47,XXX" Orphanet journal of rare diseases 5: 8 doi:101186/1750-1172-5-8 PMC 2883963  PMID 20459843 
  17. ^ Harper, JC; Harton G 2010 "The use of arrays in preimplantation genetic diagnosis and screening" Fertil Steril 94 4: 1173–1177 doi:101016/jfertnstert201004064 PMID 20579641 
  18. ^ "Karyotyping" National Institute of Health Retrieved 7 May 2014 
  19. ^ Montag, M; van der Ven, K; Rösing, B; van der Ven, H; Rösing, B; Van Der Ven, H 2009 "Polar body biopsy: a viable alternative to preimplantation genetic diagnosis and screening" Reproductive biomedicine online 18 Suppl 1: 6–11 doi:101016/s1472-64831060109-5 PMID 19281658 CS1 maint: Multiple names: authors list link
  20. ^ Parnes, YM Mar–Apr 1989 "RCT controversy" Journal of Obstetric, Gynecologic, & Neonatal Nursing 18 2: 90 doi:101111/j1552-69091989tb00470x PMID 2709181 
  21. ^ Yu, Y; Zhao, Y; Li, R; Li, L; Zhao, H; Li, M; Sha, J; Zhou, Q; Qiao, J Dec 6, 2013 "Assessment of the risk of blastomere biopsy during preimplantation genetic diagnosis in a mouse model: reducing female ovary function with an increase in age by proteomics method" Journal of Proteome Research 12 12: 5475–86 doi:101021/pr400366j PMID 24156634 
  22. ^ Shi Q, Ko E, Barclay L, Hoang T, Rademaker A, Martin R 2001 "Cigarette smoking and aneuploidy in human sperm" Mol Reprod Dev 59 4: 417–21 doi:101002/mrd1048 PMID 11468778 
  23. ^ Rubes J, Lowe X, Moore D, Perreault S, Slott V, Evenson D, Selevan SG, Wyrobek AJ 1998 "Smoking cigarettes is associated with increased sperm disomy in teenage men" Fertil Steril 70 4: 715–23 doi:101016/S0015-02829800261-1 PMID 9797104 
  24. ^ Benassi-Evans B, Fenech M 2011 "Chronic alcohol exposure induces genome damage measured using the cytokinesis-block micronucleus cytome assay and aneuploidy in human B lymphoblastoid cell lines" Mutagenesis 26 3: 421–9 doi:101093/mutage/geq110 PMID 21273273 
  25. ^ McHale CM, Smith MT, Zhang L 2014 "Application of toxicogenomic profiling to evaluate effects of benzene and formaldehyde: from yeast to human" Ann N Y Acad Sci 1310: 74–83 doi:101111/nyas12382 PMC 3978411  PMID 24571325 
  26. ^ Xia Y, Bian Q, Xu L, Cheng S, Song L, Liu J, Wu W, Wang S, Wang X 2004 "Genotoxic effects on human spermatozoa among pesticide factory workers exposed to fenvalerate" Toxicology 203 1–3: 49–60 doi:101016/jtox200405018 PMID 15363581 
  27. ^ Xia Y, Cheng S, Bian Q, Xu L, Collins MD, Chang HC, Song L, Liu J, Wang S, Wang X 2005 "Genotoxic effects on spermatozoa of carbaryl-exposed workers" Toxicol Sci 85 1: 615–23 doi:101093/toxsci/kfi066 PMID 15615886 

non disjunction, non disjunction animation, non disjunction biology, non disjunction definition biology, non disjunction examples, non disjunction in meiosis 1, non disjunction karyotype, non disjunction occurs when, non disjunction of chromosomes, non disjunction video


Nondisjunction Information about

Nondisjunction


  • user icon

    Nondisjunction beatiful post thanks!

    29.10.2014


Nondisjunction
Nondisjunction
Nondisjunction viewing the topic.
Nondisjunction what, Nondisjunction who, Nondisjunction explanation

There are excerpts from wikipedia on this article and video

Random Posts

Body politic

Body politic

The body politic is a metaphor that regards a nation as a corporate entity,2 likened to a human body...
Kakamega

Kakamega

Kakamega is a town in western Kenya lying about 30 km north of the Equator It is the headquarte...
Academic year

Academic year

An academic year is a period of time which schools, colleges and universities use to measure a quant...
Lucrezia Borgia

Lucrezia Borgia

Lucrezia Borgia Italian pronunciation: luˈkrɛttsja ˈbɔrdʒa; Valencian: Lucrècia Borja luˈkrɛsia...