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In molecular biology, LSm proteins are a family of RNA-binding proteins found in virtually every cellular organism LSm is a contraction of 'like Sm', because the first identified members of the LSm protein family were the Sm proteins LSm proteins are defined by a characteristic three-dimensional structure and their assembly into rings of six or seven individual LSm protein molecules, and play a large number of various roles in mRNA processing and regulation

The Sm proteins were first discovered as antigens targeted by so-called Anti-Sm antibodies in a patient with a form of Systemic lupus erythematosus SLE, a debilitating autoimmune disease They were named Sm proteins in honor of Stephanie Smith, a patient who suffered from SLE[1] Other proteins with very similar structures were subsequently discovered and named LSm proteins New members of the LSm protein family continue to be identified and reported

Proteins with similar structures are grouped into a hierarchy of protein families, superfamilies, and folds The LSm protein structure is an example of a small beta sheet folded into a short barrel Individual LSm proteins assemble into a six or seven member doughnut ring more properly termed a torus, which usually binds to a small RNA molecule to form a ribonucleoprotein complex The LSm torus assists the RNA molecule to assume and maintain its proper three-dimensional structure Depending on which LSm proteins and RNA molecule are involved, this ribonucleoprotein complex facilitates a wide variety of RNA processing including degradation, editing, splicing, and regulation

Alternate terms for LSm family are LSm fold and Sm-like fold, and alternate capitalization styles such as lsm, LSM, and Lsm are common and equally acceptable


  • 1 History
    • 11 Discovery of the Smith antigen
    • 12 Sm proteins, snRNPs, the spliceosome and messenger RNA splicing
    • 13 Discovery of proteins similar to the Sm proteins
    • 14 Discovery of the LSm fold
  • 2 Structure
    • 21 Secondary
    • 22 Tertiary
    • 23 Quaternary
    • 24 RNA oligonucleotide binding
  • 3 Functions
    • 31 Sm ring
    • 32 Lsm2-8 ring
    • 33 Sm10/Sm11 ring
    • 34 Lsm1-7 ring
    • 35 Gemin6 and Gemin7
    • 36 LSm12-16 and other multi-domain LSm proteins
    • 37 Archaeal Sm rings
    • 38 Bacterial LSm rings
  • 4 Evolution and phylogeny
    • 41 Homomeric LSm rings in bacteria and archaea
    • 42 Heteromeric LSm rings in eukaryotes
  • 5 Biogenesis of snRNPs
  • 6 References
  • 7 External links


Discovery of the Smith antigen

The story of the discovery of the first LSm proteins begins with a young woman, Stephanie Smith, who was diagnosed in 1959 with systemic lupus erythematosus SLE, eventually succumbing to complications of the disease in 1969 at the age of 22[1] During this period, she was treated at New York's Rockefeller University Hospital, under the care of Dr Henry Kunkel and Dr Eng Tan As those with an autoimmune disease, SLE patients produce antibodies to antigens in their cells' nuclei, most frequently to their own DNA However, Dr Kunkel and Dr Tan found in 1966 that Ms Smith produced antibodies to a set of nuclear proteins, which they named the 'smith antigen' Sm Ag[2] About 30% of SLE patients produce antibodies to these proteins, as opposed to double stranded DNA This discovery improved diagnostic testing for SLE, but the nature and function of this antigen was unknown

Sm proteins, snRNPs, the spliceosome and messenger RNA splicing

Research continued during the 1970s and early 1980s The smith antigen was found to be a complex of ribonucleic acid RNA molecules and multiple proteins A set of uridine-rich small nuclear RNA snRNA molecules was part of this complex, and given the names U1, U2, U4, U5 and U6 Four of these snRNAs U1, U2, U4 and U5 were found to be tightly bound to several small proteins, which were named SmB, SmD, SmE, SmF, and SmG in decreasing order of size SmB has an alternatively spliced variant, SmB', and a very similar protein, SmN, replaces SmB'/B in certain mostly neural tissues SmD was later discovered to be a mixture of three proteins, which were named SmD1, SmD2 and SmD3 These nine proteins SmB, SmB', SmN, SmD1, SmD2, SmD3, SmE, SmF and SmG became known as the Sm core proteins, or simply Sm proteins The snRNAs are complexed with the Sm core proteins and with other proteins to form particles in the cell's nucleus called small nuclear ribonucleoproteins, or snRNPs By the mid 1980s, it became clear that these snRNPs help form a large 48 MD molecular weight complex, called the spliceosome, around pre-mRNA, excising portions of the pre-mRNA called introns and splicing the coding portions exons together[3] After a few more modifications, the spliced pre-mRNA becomes messenger RNA mRNA which is then exported from the nucleus and translated into a protein by ribosomes

Discovery of proteins similar to the Sm proteins

The snRNA U6 unlike U1, U2, U4 and U5 does not associate with the Sm proteins, even though the U6 snRNP is a central component in the spliceosome In 1999 a protein heteromer was found that binds specifically to U6, and consisted of seven proteins clearly homologous to the Sm proteins These proteins were denoted LSm like Sm proteins LSm1, LSm2, LSm3, LSm4, LSm5, LSm6 and LSm7, with the similar LSm8 protein identified later In the bacterium Escherichia coli, the Sm-like protein HF-I encoded by the gene hfq was described in 1968 as an essential host factor for RNA bacteriophage Qβ replication The genome of Saccharomyces cerevisiae Baker's Yeast was sequenced in the mid-1990s, providing a rich resource for identifying homologs of these human proteins Subsequently, as more eukaryotes genomes were sequenced, it became clear that eukaryotes, in general, share homologs to the same set of seven Sm and eight LSm proteins[4] Soon after, proteins homologous to these eukaryote LSm proteins were found in Archaea Sm1 and Sm2 and Bacteria Hfq and YlxS homologs[5] Interestingly, the archaeal LSm proteins are more similar to the eukaryote LSm proteins than either are to bacterial LSm proteins The LSm proteins described thus far were rather small proteins, varying from 76 amino acids 87 kD molecular weight for human SmG to 231 amino acids 29 kD molecular weight for human SmB But recently, larger proteins have been discovered that include a LSm structural domain in addition to other protein structural domains such as LSm10, LSm11, LSm12, LSm13, LSm14, LSm15, LSm16, ataxin-2, as well as archaeal Sm3

Discovery of the LSm fold

Around 1995, comparisons between the various LSm homologs identified two sequence motifs, 32 nucleic acids long 14 amino acids, that were very similar in each LSm homolog, and were separated by a non-conserved region of variable length This indicated the importance of these two sequence motifs named Sm1 and Sm2, and suggested that all LSm protein genes evolved from a single ancestral gene[6] In 1999, crystals of recombinant Sm proteins were prepared, allowing X-ray crystallography and determination of their atomic structure in three dimensions[7] This demonstrated that the LSm proteins share a similar three-dimensional fold of a short alpha helix and a five-stranded folded beta sheet, subsequently named the LSm fold Other investigations found that LSm proteins assemble into a torus doughnut-shaped ring of six or seven LSm proteins, and that RNA binds to the inside of the torus, with one nucleotide bound to each LSm protein


LSm secondary structure showing the N-terminal alpha helix and the five-strand antiparallel beta sheet The LSm protein Human SmD1 showing the eight-strand beta sandwich peptide backbone description The beta sheet fold hinge runs along the bottom of the image

Uridine phosphate binds in archaeal Sm1 between the β2b/β3a loop and β4b/β5 loop The uracil is stacked between the histidine and arginine residues, stabilized by hydrogen bonding to an asparagine residue, and hydrogen bonding between the aspartate residue and the ribose LSm proteins are characterized by a beta sheet the secondary structure, folded into the LSm fold the tertiary structure, polymerization into a six or seven member torus the quaternary structure, and binding to RNA oligonucleotides[8] A modern paradigm classifies proteins on the basis of protein structure and is a currently active field, with three major approaches, SCOP Structural Classification of Proteins, CATH Class, Architecture, Topology, Homologous superfamily, and FSSP/DALI Families of Structurally Similar Proteins


The secondary structure of a LSm protein is a small five-strand anti-parallel beta sheet, with the strands identified from the N-terminal end to the C-terminal end as β1, β2, β3, β4, β5 The SCOP class of All beta proteins and the CATH class of Mainly Beta are defined as protein structures that are primarily beta sheets, thus including LSm The SM1 sequence motif corresponds to the β1, β2, β3 strands, and the SM2 sequence motif corresponds to the β4 and β5 strands The first four beta strands are adjacent to each other, but β5 is adjacent to β1, turning the overall structure into a short barrel This structural topology is described as 51234 A short two to four turns N-terminal alpha helix is also present in most LSm proteins The β3 and β4 strands are short in some LSm proteins, and are separated by an unstructured coil of variable length The β2, β3 and β4 strands are strongly bent about 120° degrees at their midpoints The bends in these strands are often glycine, and the side chains internal to the beta barrel are often the hydrophobic residues valine, leucine, isoleucine and methionine


SCOP simply classifies the LSm structure as the Sm-like fold, one of 149 different Beta Protein folds, without any intermediate groupings The LSm beta sheet is sharply bent and described as a Roll architecture in CATH one of 20 different beta protein architectures in CATH One of the beta strands β5 in LSm crosses the open edge of the roll to form a small SH3 type barrel topology one of 33 beta roll topologies in CATH CATH lists 23 homologous superfamilies with an SH3 type barrel topology, one of which is the LSm structure RNA Binding Protein in the CATH system SCOP continues its structural classification after Fold to Superfamily, Family and Domain, while CATH continues to Sequence Family, but these divisions are more appropriately described in the "Evolution and phylogeny" section

The SH3-type barrel tertiary structure of the LSm fold is formed by the strongly bent about 120° β2, β3 and β4 strands, with the barrel structure closed by the β5 strand Emphasizing the tertiary structure, each bent beta strand can be described as two shorter beta strands The LSm fold can be viewed as an eight-strand anti-parallel beta sandwich, with five strands in one plane and three strands in a parallel plane with about a 45° pitch angle between the two halves of the beta sandwich The short two to four turns N-terminal alpha helix occurs at one edge of the beta sandwich This alpha helix and the beta strands can be labeled from the N-terminus to the C-terminus α, β1, β2a, β2b, β3a, β3b, β4a, β4b, β5 where the a and b refer to either the two halves of a bent strand in the five-strand description, or to the individual strands in the eight-strand description Each strand in the eight-strand description is formed from five amino acid residues Including the bends and loops between the strands, and the alpha helix, about 60 amino acid residues contribute to the LSm fold, but this varies between homologs due to variation in inter-strand loops, the alpha helix, and even the lengths of β3b and β4a strands


LSm proteins typically assemble into a LSm ring, a six or seven member torus, about 7 nanometers in diameter with a 2 nanometer hole The ancestral condition is a homohexamer or homoheptamer of identical LSm subunits LSm proteins in eukaryotes form heteroheptamers of seven different LSm subunits, such as the Sm proteins Binding between the LSm proteins is best understood with the eight-strand description of the LSm fold The five-strand half of the beta sandwich of one subunit aligns with the three-strand half of the beta sandwich of the adjacent subunit, forming a twisted 8-strand beta sheet Aβ4a/Aβ3b/Aβ2a/Aβ1/Aβ5/Bβ4b/Bβ3a/Bβ2b, where the A and B refer to the two different subunits In addition to hydrogen bonding between the Aβ5 and Bβ4b beta strands of the two LSm protein subunits, there are energetically favorable contacts between hydrophobic amino acid side chains in the interior of the contact area, and energetically favorable contacts between hydrophilic amino acid side chains around the periphery of the contact area

RNA oligonucleotide binding

LSm rings form ribonucleoprotein complexes with RNA oligonucleotides that vary in binding strength from very stable complexes such as the Sm class snRNPs to transient complexes RNA oligonucleotides generally bind inside the hole lumen of the LSm torus, one nucleotide per LSm subunit, but additional nucleotide binding sites have been reported at the top α helix side of the ring The exact chemical nature of this binding varies, but common motifs include stacking the heterocyclic base often uracil between planar side chains of two amino acids, hydrogen bonding to the heterocyclic base and/or the ribose, and salt bridges to the phosphate group


The various kinds of LSm rings function as scaffolds or chaperones for RNA oligonucleotides, assisting the RNA to assume and maintain the proper three-dimensional structure In some cases, this allows the oligonucleotide RNA to function catalytically as a ribozyme In other cases, this facilitates modification or degradation of the RNA, or the assembly, storage, and intracellular transport of ribonucleoprotein complexes[9]

Sm ring

The Sm ring is found in the nucleus of all eukaryotes about 25 x 106 copies per proliferating human cell, and has the best understood functions The Sm ring is a heteroheptamer The Sm-class snRNA molecule in the 5' to 3' direction enters the lumen doughnut hole at the SmE subunit and proceeds sequentially in a clockwise fashion looking from the α helix side inside the lumen doughnut hole to the SmG, SmD3, SmB, SmD1, SmD2 subunits, exiting at the SmF subunit[10] SmB can be replaced by the splice variant SmB' and by SmN in neural tissues The Sm ring permanently binds to the U1, U2, U4 and U5 snRNAs which form four of the five snRNPs that constitute the major spliceosome The Sm ring also permanently binds to the U11, U12 and U4atac snRNAs which form four of the five snRNPs including the U5 snRNP that constitute the minor spliceosome Both of these spliceosomes are central RNA-processing complexes in the maturation of messenger RNA from pre-mRNA Sm proteins have also been reported to be part of ribonucleoprotein component of telomerase[11]

Lsm2-8 ring

The two Lsm2-8 snRNPs U6 and U6atac have the key catalyic function in the major and minor spliceosomes These snRNPs do not include the Sm ring, but instead use the heteroheptameric Lsm2-8 ring The LSm rings are about 20 times less abundant than the Sm rings The order of these seven LSm proteins in this ring is not known, but based on amino acid sequence homology with the Sm proteins, it is speculated that the snRNA in the 5' to 3' direction may bind first to LSm5, and precedes sequentially clockwise to the LSm7, LSm4, LSm8, LSm2, LSm3, and exiting at the LSm6 subunit Experiments with Saccharomyces cerevisiae budding yeast mutations suggest that the Lsm2-8 ring assists the reassociation of the U4 and U6 snRNPs into the U4/U6 di-snRNP[12] After completion of exon deletion and intron splicing, these two snRNPs must reassociate for the spliceosome to initiate another exon/intron splicing cycle In this role, the Lsm2-8 ring acts as an RNA chaperone instead of an RNA scaffold The Lsm2-8 ring also forms an snRNP with the U8 small nucleolar RNA snoRNA which localizes in the nucleolus This ribonucleoprotein complex is necessary for processing ribosomal RNA and transfer RNA to their mature forms[13] The Lsm2-8 ring is reported to have a role in the processing of pre-P RNA into RNase P RNA In contrast to the Sm ring, the Lsm2-8 ring does not permanently bind to its snRNA and snoRNA

Sm10/Sm11 ring

A second type of Sm ring exists where LSm10 replaces SmD1 and LSm11 replaces SmD2 LSm11 is a two domain protein with the C-terminal domain being a LSm domain This heteroheptamer ring binds with the U7 snRNA in the U7 snRNP The U7 snRNP mediates processing of the 3' UTR stem-loop of the histone mRNA in the nucleus[14] Like the Sm ring, it is assembled in the cytoplasm onto the U7 snRNA by a specialized SMN complex

Lsm1-7 ring

A second type of Lsm ring is the Lsm1-7 ring, which has the same structure as the Lsm2-8 ring except that LSm1 replaces LSm8 In contrast to the Lsm2-8 ring, the Lsm1-7 ring localizes in the cytoplasm where it assists in degrading messenger RNA in ribonucleoprotein complexes This process controls the turnover of messenger RNA so that ribosomal translation of mRNA to protein responds quickly to changes in transcription of DNA to messenger RNA by the cell

Gemin6 and Gemin7

See also: SnRNP § Assembly of core snRNPs in the SMN complex

The SMN complex described under "Biogenesis of snRNPs" is composed of the SMN protein and Gemin2-8 Two of these, Gemin 6 and Gemin7 have been discovered to have the LSm structure, and to form a heterodimer These may have a chaperone function in the SMN complex to assist the formation of the Sm ring on the Sm-class snRNAs[15] PRMT5 complex is composed of PRMT5, pICln, WD45 Mep50 pICln helps to form Sm opened ring on SMN complex SMN complex assists in the assembly of snRNPs where the Sm ring is in the open conformation on SMN complex and this Sm ring is loaded onto the snRNA by SMN complex[16]

LSm12-16 and other multi-domain LSm proteins

The LSm12-16 proteins have been described very recently These are two-domain proteins with a N-terminal LSm domain and a C-terminal methyl transferase domain[17] Very little is known about the function of these proteins, but presumably they are member of LSm-domain rings that interact with RNA There is some evidence that LSm12 is possibly involved in mRNA degradation and LSm13-16 may have roles in regulation of mitosis A large protein of unknown function, ataxin-2, associated with the neurodegenerative disease spinocerebellar ataxia type 2, also has a N-terminal LSm domain

Archaeal Sm rings

Two LSm proteins are found in a second domain of life, the Archaea These are the Sm1 and Sm2 proteins not to be confused with the Sm1 and Sm2 sequence motifs, and are sometimes identified as Sm-like archaeal proteins SmAP1 and SmAP2 for this reason[18] Sm1 and Sm2 generally form homoheptamer rings, although homohexamer rings have been observed Sm1 rings are similar to eukaryote Lsm rings in that they form in the absence of RNA while Sm2 rings are similar to eukaryote Sm rings in that they require uridine-rich RNA for their formation They have been reported to associate with RNase P RNA, suggesting a role in transfer RNA processing, but their function in archaea in this process and possibly processing other RNA such as ribosomal RNA is mostly unknown One of the two main branches of archaea, the crenarchaeotes have a third known type of archaeal LSm protein, Sm3 This is a two-domain protein with a N-terminal LSm domain that forms a homoheptamer ring Nothing is known about the function of this LSm protein, but presumably it interacts with, and probably helps process, RNA in these organisms

Bacterial LSm rings

Several LSm proteins have been reported in the third domain of life, the Bacteria Hfq protein forms homohexamer rings, and was originally discovered as necessary for infection by the bacteriophage Qβ, although this is clearly not the native function of this protein in bacteria It is not universally present in all bacteria, but has been found in Proteobacteria, Firmicutes, Spirochaetes, Thermotogae, Aquificae and one species of Archaea This last instance is probably a case of horizontal gene transfer Hfq is pleiotropic with a variety of interactions, generally associated with translation regulation These include blocking ribosome binding to mRNA, marking mRNA for degradation by binding to their poly-A tails, and association with bacterial small regulatory RNAs such as DsrA RNA that control translation by binding to certain mRNAs[19][20] A second bacterial LSm protein is YlxS sometimes also called YhbC, which was first identified in the soil bacterium Bacillus subtilis This is a two-domain protein with a N-terminal LSm domain Its function is unknown, but amino acid sequence homologs are found in virtually every bacterial genome to date, and it may be an essential protein[21] The middle domain of the small conductance mechanosensitive channel MscS in Escherichia coli forms a homoheptameric ring[22] This LSm domain has no apparent RNA-binding function, but the homoheptameric torus is part of the central channel of this membrane protein

Evolution and phylogeny

LSm homologs are found in all three domains of life, and may even be found in every single organism Computational phylogenetic methods are used to infer phylogenetic relations Sequence alignment between the various LSm homologs are the appropriate tool for this, such as multiple sequence alignment of the primary structure amino acid sequence, and structural alignment of the tertiary structure three-dimensional structure It is hypothesized that a gene for a LSm protein was present in the last universal ancestor of all life[23] Based on the functions of known LSm proteins, this original LSm protein may have assisted ribozymes in the processing of RNA for synthesizing proteins as part of the RNA world hypothesis of early life According to this view, this gene was passed from ancestor to descendent, with frequent mutations, gene duplications and occasional horizontal gene transfers In principle, this process can be summarized in a phylogenetic tree with the root in the last universal ancestor or earlier, and with the tips representing the universe of LSm genes existing today

Homomeric LSm rings in bacteria and archaea

Based on structure, the known LSm proteins divide into a group consisting of the bacterial LSm proteins Hfq, YlxS and MscS and a second group of all other LSm proteins, in accordance with the most recently published phylogenetic trees[24] The three archaeal LSm proteins Sm1, Sm2 and Sm3 also cluster as a group, distinct from the eukaryote LSm proteins Both the bacterial and archaeal LSm proteins polymerize to homomeric rings, which is the ancestral condition

Heteromeric LSm rings in eukaryotes

A series of gene duplications of a single eukaryote LSm gene resulted in most if not all of the known eukaryote LSm genes Each of the seven Sm proteins has greater amino acid sequence homology to a corresponding Lsm protein than to the other Sm proteins This suggests that an ancestral LSm gene duplicated several times, resulting in seven paralogs These subsequently diverged from each other so that the ancestral homoheptamer LSm ring became a heteroheptamer ring Based on the known functions of LSm proteins in eukaryotes and archaea, the ancestral function may have been processing of pre-ribosomal RNA, pre-transfer RNA, and pre-RNase P Then, according to this hypothesis, the seven ancestral eukaryote LSm genes duplicated again to seven pairs of Sm/LSm paralogs; LSm1/SmB, LSm2/SmD1, LSm3/SmD2, LSm4/SmD3, LSm5/SmE, LSm6/SmF and LSm7/SmG These two group of seven LSm genes and the corresponding two kinds of LSm rings evolved to an Sm ring requiring RNA and a Lsm ring which forms without RNA The LSm1/LSm8 paralog pair also seems to have originated prior to the last common eukaryote ancestor, for a total of at least 15 LSm protein genes The SmD1/LSm10 paralog pair and the SmD2/LSm11 paralog pair exist only in animals, fungi, and the amoebozoa sometimes identified as the unikont clade and appears to be absent in the bikont clade chromalveolates, excavates, plants and rhizaria Therefore, these two gene duplications predated this fundamental split in the eukaryote lineage The SmB/SmN paralog pair is seen only in the placental mammals, which dates this LSm gene duplication

Biogenesis of snRNPs

Main article: snRNP

Small nuclear ribonucleoproteins snRNPs assemble in a tightly orchestrated and regulated process that involves both the cell nucleus and cytoplasm[25]


  1. ^ a b Reeves WH, Narain S, Satoh M 2003 "Henry Kunkel, Stephanie Smith, clinical immunology, and split genes" Lupus 12 3: 213–7 doi:101191/0961203303lu360xx PMID 12708785 
  2. ^ Tan EM, Kunkel HG March 1966 "Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus" J Immunol 96 3: 464–71 PMID 5932578 
  3. ^ Will CL, Lührmann R June 2001 "Spliceosomal UsnRNP biogenesis, structure and function" Curr Opin Cell Biol 13 3: 290–301 doi:101016/S0955-06740000211-8 PMID 11343899 
  4. ^ He W, Parker R June 2000 "Functions of Lsm proteins in mRNA degradation and splicing" Curr Opin Cell Biol 12 3: 346–50 doi:101016/S0955-06740000098-3 PMID 10801455 
  5. ^ Törö I, Thore S, Mayer C, Basquin J, Séraphin B, Suck D May 2001 "RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex" EMBO J 20 9: 2293–303 doi:101093/emboj/2092293 PMC 125243  PMID 11331594 
  6. ^ Hermann H, Fabrizio P, Raker VA, Foulaki K, Hornig H, Brahms H, Lührmann R May 1995 "snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions" EMBO J 14 9: 2076–88 PMC 398308  PMID 7744013 
  7. ^ Kambach C, Walke S, Young R, Avis JM, de la Fortelle E, Raker VA, Lührmann R, Li J, Nagai K February 1999 "Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs" Cell 96 3: 375–87 doi:101016/S0092-86740080550-4 PMID 10025403 
  8. ^ National Center for Biotechnology Information Structure Database PDB codes 1B34, 1D3B, 1I5L, 1KQ2, 1N9S, 1IB8
  9. ^ Khusial P, Plaag R, Zieve GW September 2005 "LSm proteins form heptameric rings that bind to RNA via repeating motifs" Trends Biochem Sci 30 9: 522–8 doi:101016/jtibs200507006 PMID 16051491 
  10. ^ Urlaub H, Raker VA, Kostka S, Lührmann R January 2001 "Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure" EMBO J 20 1-2: 187–96 doi:101093/emboj/201187 PMC 140196  PMID 11226169 
  11. ^ Seto AG, Zaug AJ, Sobel SG, Wolin SL, Cech TR September 1999 "Saccharomyces cerevisiae telomerase is an Sm small nuclear ribonucleoprotein particle" Nature 401 6749: 177–80 doi:101038/43694 PMID 10490028 
  12. ^ Beggs JD June 2005 "Lsm proteins and RNA processing" Biochem Soc Trans 33 Pt 3: 433–8 doi:101042/BST0330433 PMID 15916535 
  13. ^ Kufel J, Allmang C, Petfalski E, Beggs J, Tollervey D January 2003 "Lsm Proteins are required for normal processing and stability of ribosomal RNAs" J Biol Chem 278 4: 2147–56 doi:101074/jbcM208856200 PMID 12438310 
  14. ^ Schümperli D, Pillai RS October 2004 "The special Sm core structure of the U7 snRNP: far-reaching significance of a small nuclear ribonucleoprotein" Cell Mol Life Sci 61 19-20: 2560–70 doi:101007/s00018-004-4190-0 PMID 15526162 
  15. ^ Ma Y, Dostie J, Dreyfuss G, Van Duyne GD June 2005 "The Gemin6-Gemin7 heterodimer from the survival of motor neurons complex has an Sm protein-like structure" Structure 13 6: 883–92 doi:101016/jstr200503014 PMID 15939020 
  16. ^ Chari A, Golas MM, Klingenhäger M, Neuenkirchen N, Sander B, Englbrecht C, Sickmann A, Stark H, Fischer U 2008-10-31 "An Assembly Chaperone Collaborates with the SMN Complex to Generate Spliceosomal SnRNPs" Cell 135 3: 497–509 doi:101016/jcell200809020 PMID 18984161 
  17. ^ Albrecht M, Lengauer T July 2004 "Novel Sm-like proteins with long C-terminal tails and associated methyltransferases" FEBS Lett 569 1-3: 18–26 doi:101016/jfebslet200403126 PMID 15225602 
  18. ^ Mura C, Kozhukhovsky A, Gingery M, Phillips M, Eisenberg D April 2003 "The oligomerization and ligand-binding properties of Sm-like archaeal proteins SmAPs" Protein Sci 12 4: 832–47 doi:101110/ps0224703 PMC 2323858  PMID 12649441 
  19. ^ Schumacher MA, Pearson RF, Møller T, Valentin-Hansen P, Brennan RG July 2002 "Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein" EMBO J 21 13: 3546–56 doi:101093/emboj/cdf322 PMC 126077  PMID 12093755 
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  21. ^ Yu L, Gunasekera AH, Mack J, Olejniczak ET, Chovan LE, Ruan X, Towne DL, Lerner CG, Fesik SW August 2001 "Solution structure and function of a conserved protein SP143 encoded by an essential Streptococcus pneumoniae gene" J Mol Biol 311 3: 593–604 doi:101006/jmbi20014894 PMID 11493012 
  22. ^ Bass RB, Strop P, Barclay M, Rees DC November 2002 "Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel" Science 298 5598: 1582–7 doi:101126/science1077945 PMID 12446901 
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External links

  • Pfam entry LSM Pfam is the Sanger Institute database, which is a collection of protein families and domains

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