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Cell-free fetal DNA

cell-free fetal dna testing, cell-free fetal dna analysis
Cell-free fetal DNA cffDNA is fetal DNA which circulates freely in the maternal blood Maternal blood is sampled by venipuncture Analysis of cffDNA is a method of non-invasive prenatal diagnosis frequently ordered for pregnant women of advanced maternal age Two hours after delivery, cffDNA is no longer detectable in maternal blood

Contents

  • 1 Background
  • 2 Laboratory methods
    • 21 Separation of cffDNA
    • 22 Analysis of cffDNA
      • 221 Quantitative real-time PCR
      • 222 Nested PCR
      • 223 Digital PCR
      • 224 Shotgun sequencing
      • 225 Mass spectrometry
      • 226 Epigenetic modifications
      • 227 mRNA
  • 3 Applications
    • 31 Prenatal sex discernment
    • 32 Congenital adrenal hyperplasia
    • 33 Paternity testing
    • 34 Single gene disorders
    • 35 Hemolytic disease of the fetus and newborn
    • 36 Aneuploidy
    • 37 Preeclampsia
  • 4 Future perspectives
  • 5 See also
  • 6 References

Background

Cell free fetal DNA sheds into the maternal blood circulation

cffDNA originates from placental trophoblasts[1][2] Fetal DNA is fragmented when placental microparticles are shed into the maternal blood circulation[3]

cffDNA fragments are approximately 200 base pairs bp in length They are significantly smaller than maternal DNA fragments[4] The difference in size allows cffDNA to be distinguished from maternal DNA fragments[5][6]

Approximately 11 to 134 percent of the cell-free DNA in maternal blood is of fetal origin The amount varies widely from one pregnant woman to another[7] cffDNA is present after five to seven weeks gestation The amount of cffDNA increases as the pregnancy progresses[8] The quantity of cffDNA in maternal blood diminishes rapidly after childbirth Two hours after delivery, cffDNA is no longer detectable in maternal blood[9]

Analysis of cffDNA may provide earlier diagnosis of fetal conditions than current techniques As cffDNA is found in maternal blood, sampling carries no associated risk of spontaneous abortion[10][11][12][13][14] cffDNA analysis has the same ethical and practical issues as other techniques such as amniocentesis and chorionic villus sampling[15]

Some disadvantages of sampling cffDNA include a low concentration of cffDNA in maternal blood; variation in the quantity of cffDNA between individuals; a high concentration of maternal cell free DNA compared to the cffDNA in maternal blood[16]

New evidence shows that cffDNA test failure rate is higher, fetal fraction proportion of fetal versus maternal DNA in the maternal blood sample is lower and PPV for trisomies 18, 13 and SCA is decreased in IVF pregnancies compared to those conceived spontaneously[17]

Laboratory methods

A number of laboratory methods have been developed for cell-free fetal DNA screening for genetic defects have been developed The main ones are 1 massively parallel shotgun sequencing MPSS, 2 targeted massive parallel sequencing t-MPS and 3 single nucleotide polymorphism SNP based approach[18][19][20]

A maternal peripheral blood sample is taken by venesection at about ten weeks gestation[21]

Separation of cffDNA

Blood plasma is separated from the maternal blood sample using a laboratory centrifuge The cffDNA is then isolated and purified[22] A standardized protocol for doing this was written through an evaluation of the scientific literature The highest yield in cffDNA extraction was obtained with the "QIAamp DSP Virus Kit"[23]

Addition of formaldehyde to maternal blood samples increases the yield of cffDNA Formaldehyde stabilizes intact cells, and therefore inhibits the further release of maternal DNA With the addition of formaldehyde, the percentage of cffDNA recovered from a maternal blood sample varies between 032 percent and 40 percent with a mean of 77 percent[24] Without the addition of formaldehyde, the mean percentage of cffDNA recovered has been measured at 202 percent However, other figures vary between 5 and 96 percent[25][26]

Recovery of cffDNA may be related to the length of the DNA fragments Another way to increase the fetal DNA is based on physical length of DNA fragments Smaller fragments can represent up to seventy percent of the total cell free DNA in the maternal blood sample

Analysis of cffDNA

In real-time PCR, fluorescent probes are used to monitor the accumulation of amplicons The reporter fluorescent signal is proportional to the number of amplicons generated The most appropriate real time PCR protocol is designed according to the particular mutation or genotype to be detected Point mutations are analysed with qualitative real time PCR with the use of allele specific probes insertions and deletions are analyzed by dosage measurements using quantitative real time PCR

cffDNA may be detected by finding paternally inherited DNA sequences via polymerase chain reaction PCR[27][28]

Quantitative real-time PCR

sex-determining region Y gene SRY and Y chromosome short tandem repeat "DYS14" in cffDNA from 511 pregnancies were analyzed using quantitative real-time PCR RT-qPCR In 401 of 403 pregnancies where maternal blood was drawn at seven weeks gestation or more, both segments of DNA were found[29]

Nested PCR

The use of nested polymerase chain reaction nested PCR was evaluated to determine sex by detecting a Y chromosome specific signal in the cffDNA from maternal plasma Nested PCR detected 53 of 55 male fetuses The cffDNA from the plasma of 3 of 25 women with female fetuses contained the Y chromosome-specific signal The sensitivity of nested PCR in this experiment was 96 percent The specificity was 88 percent[30]

Digital PCR

Microfluidic devices allow the quantification of cffDNA segments in maternal plasma with accuracy beyond that of real-time PCR Point mutations, loss of heterozygosity and aneuploidy can be detected in a single PCR step[31][32][33] Digital PCR can differentiate between maternal blood plasma and fetal DNA in a multiplex fashion[31]

Shotgun sequencing

High throughput shotgun sequencing using tools such as Solexa or Illumina, yields approximately 5 million sequence tags per sample of maternal serum Aneuploid pregnancies such as trisomy where identified when testing at the fourteenth week of gestation Fetal whole of genome mapping by parental haplotype analysis was completed using sequencing of cffDNA from maternal serum[13] Pregnant females were studied using a 2-plex massively parallel maternal plasma DNA sequencing and trisomy was diagnosed with z-score greater than 3[34] The sequencing gave sensitivity of 100 percent, specificity of 979 percent, a positive predictive value of 966 percent and a negative predictive value of 100 percent

Mass spectrometry

Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry MALDI-TOF MS combined with single-base extension after PCR allows cffDNA detection with single base specificity and single DNA molecule sensitivity[35] DNA is amplified by PCR Then, linear amplification with base extension reaction with a third primer is designed to anneal to the region upstream from the mutation site One or two bases are added to the extension primer to produce two extension products from wild-type DNA and mutant DNA Single base specificity provides advantages over hybridization-based techniques using TaqMan hydrolysis probes When assessing the technique, no false positives or negatives were found when looking for cffDNA to determine fetal sex in sixteen maternal plasma samples[35] The sex of ninety of ninety-one male foetuses were correctly detected using MALDI-TOF mas spectrometry The technique had accuracy, sensitivity and specificity of over 99 percent[36]

Epigenetic modifications

Differences in gene activation between maternal and fetal DNA can be exploited epigenetic modifications heritable modifications that change gene function without changing DNA sequence can be used to detect cffDNA[37][38] The hypermethylated RASSF1A promoter is a universal fetal marker used to confirm the presence of cffDNA[39] A technique was described where cffDNA was extracted from maternal plasma and then digested with methylation-sensitive and insensitive restriction enzymes Then, real-time PCR analysis of RASSF1A, SRY, and DYS14 was done[39] The procedure detected 79 out of 90 88 percent maternal blood samples where hypermethylated RASSF1A was present

mRNA

mRNA transcripts from genes expressed in the placenta are detectable in maternal plasma[40] In this procedure, plasma is centrifuged so an aqueous layer appears This layer is transferred and from it RNA is extracted RT-PCR is used to detect a selected expression of RNA For example, Human placental lactogen hPL and beta-hCG mRNA are stable in maternal plasma and can be detected Ng et al 2002 This can help to confirm the presence of cffDNA in maternal plasma[16]

Applications

Prenatal sex discernment

Further information: Prenatal sex discernment

The analysis of cffDNA from a sample of maternal plasma allows for prenatal sex discernment Applications of prenatal sex discernment include:

  • Disease testing: Whether the sex of the fetus is male or female allows the determination of the risk of a particular X-linked recessive genetic disorder in a particular pregnancy, especially where the mother is a genetic carrier of the disorder[41]
  • Preparation, for any sex-dependent aspects of parenting
  • Sex selection, which after preimplantation genetic diagnosis may be performed by selecting only embryos of the preferred sex, or, after post-implantation methods by performing sex-selective abortion depending on the test result and personal preference

In comparison to obstetric ultrasonography which is unreliable for sex determination in the first trimester and amniocentesis which carries a small risk of miscarriage, sampling of maternal plasma for analysis of cffDNA is without risk[42] The main targets in the cffDNA analysis are the gene responsible for the sex-determining region Y protein SRY on the Y chromosome and the DYS14 sequence[43][44]

Congenital adrenal hyperplasia

In congenital adrenal hyperplasia, the adrenal cortex lacks appropriate corticosteroid synthesis, leading to excess adrenal androgens and affects female fetuses[45] There is an external masculinization of the genitalia in the female fetuses[46] Mothers of at risk fetuses are given dexamethasone at 6 weeks gestation to suppress pituitary gland release of androgens[47]

If analysis of cffDNA obtained from a sample of maternal plasma lacks genetic markers found only on the Y chromosome, it is suggestive of a female fetus However, it might also indicate a failure of the analysis itself a false negative result Paternal genetic polymorphisms and sex-independent markers may be used to detect cffDNA An high degree of heterozygosity of these markers must be present for this application[48]

Paternity testing

Prenatal DNA paternity testing is commercially available The test can be performed at nine weeks gestation[citation needed]

Single gene disorders

Autosomal dominant and recessive single gene disorders which have been diagnosed prenatally by analysing paternally inherited DNA include cystic fibrosis, beta thalassemia, sickle cell anemia, spinal muscular atrophy, and myotonic dystrophy[27][43] Prenatal diagnosis of single gene disorders which are due to an autosomal recessive mutation, a maternally inherited autosomal dominant mutation or large sequence mutations that include duplication, expansion or insertion of DNA sequences is more difficult[49]

In cffDNA, fragments of 200 – 300 bp length involved in single gene disorders are more difficult to detect

For example, the autosomal dominant condition, achondroplasia is caused by the FGFR3 gene point mutation[50] In two pregnancies with a fetus with achondroplasia was found a paternally inherited G1138A mutation from cffDNA from a maternal plasma sample in one and a G1138A de novo mutation from the other[50]

In studies of the genetics of Huntington's chorea using qRT-PCR of cffDNA from maternal plasma samples, CAG repeats have been detected at normal levels 17, 20 and 24[51]

cffDNA may also be used to diagnose single gene disorders[15] Developments in laboratory processes using cffDNA may allow prenatal diagnosis of aneuploidies such as trisomy 21 Down's syndrome in the fetus[52][32]

Hemolytic disease of the fetus and newborn

Incompatibility of fetal and maternal RhD antigens is the main cause of Hemolytic disease of the newborn[53] Approximately 15 percent of Caucasian women, 3 to 5 percent of black Africa women and less than 3 percent of Asian women are RhD negative[54]

Accurate prenatal diagnosis is important because the disease can be fatal to the newborn and because treatment including intramuscular immunoglobulin Anti-D or intravenous immunoglobulin can be administered to mothers at risk[55]

PCR to detect RHD gene gene exons 5 and 7 from cffDNA obtained from maternal plasma between 9 and 13 weeks gestation gives a high degree of specificity, sensitivity and diagnostic accuracy >90 percent when compared to RhD determination from newborn cord blood serum[53] Similar results were obtained targeting exons 7 and 10[56] Droplet digital PCR in fetal RhD determination was comparable to a routine real-time PCR technique[57]

Routine determination of fetal RhD status from cffDNA in maternal serum allows early management of at risk pregnancies while decreasing unnecessary use of Anti-D by over 25 percent[58]

Aneuploidy

Sex chromosomes

Analysis of maternal serum cffDNA by high-throughput sequencing can detect common fetal sex chromosome aneuploidies such as Turner's syndrome, Klinefelter's syndrome and triple X syndrome but the procedure's positive predictive value is low[59]

Trisomy 21

Fetal trisomy of chromosome 21 is the cause of Down's syndrome This trisomy can be detected by analysis of cffDNA from maternal blood by massively parallel shotgun sequencing MPSS[60] Another technique is digital analysis of selected regions DANSR[60] However, such tests show inconsistent degrees of sensitivity and specificity and therefore may be best used to confirm a positive maternal screening test such as ultrasound markers of the condition[60][61]

Trisomy 13 and 18

Analysis of cffDNA from maternal plasma with MPSS looking for trisomy 13 or 18 is possible[62]

Factors limiting sensitivity and specificity include the levels of cffDNA in the maternal plasma; maternal chromosomes may have mosaicism[63]

A number of fetal nucleic acid molecules derived from aneuploid chromosomes can be detected including SERPINEB2 mRNA, clad B, hypomethylated SERPINB5 from chromosome 18, placenta-specific 4 PLAC4, hypermethylated holocarboxylase synthetase HLCS and c21orf105 mRNA from chromosome 12[64] With complete trisomy, the mRNA alleles in maternal plasma isn't the normal 1:1 ratio, but is in fact 2:1 Allelic ratios determined by epigenetic markers can also be used to detect the complete trisomies Massive parallel sequencing and digital PCR for fetal aneuploidy detection can be used without restriction to fetal-specific nucleic acid molecules MPSS is estimated to have a sensitivity of between 96 and 100%, and a specificity between 94 and 100% for detecting Down syndromeIt can be performed at 10 weeks of gestational age[65] One study in the United States estimated a false positive rate of 03% and a positive predictive value of 80% when using cffDNA to detect Down syndrome[66]

Preeclampsia

Preeclampsia is a complex condition of pregnancy involving hypertension and proteinuria usually after 20 weeks gestation[67] It is associated with poor cytotrophoblastic invasion of the myometrium Onset of the condition between 20 and 34 weeks gestation, is considered "early"[68] Maternal plasma samples in pregnancies complicated by preeclampsia have significantly higher levels of cffDNA that those in normal pregnancies[69][70][71] This holds true for early onset preeclampsia[68]

Future perspectives

New generation sequencing may be used to yield a whole genome sequence from cffDNA This raises ethical questions[72] However, the utility of the procedure may increase as clear associations between specific genetic variants and disease states are discovered[73][74]

See also

  • Triple test
  • Quad test

References

  1. ^ Alberry M, Maddocks D, Jones M, Abdel Hadi M, Abdel-Fattah S, Avent N, Soothill PW May 2007 "Free fetal DNA in maternal plasma in anembryonic pregnancies: confirmation that the origin is the trophoblast" Prenatal Diagnosis Wiley-Blackwell 27 5: 415–8 doi:101002/pd1700 PMID 17286310 
  2. ^ Gupta AK, Holzgreve W, Huppertz B, Malek A, Schneider H, Hahn S November 2004 "Detection of fetal DNA and RNA in placenta-derived syncytiotrophoblast microparticles generated in vitro" Clinical Chemistry American Association for Clinical Chemistry AACC 50 11: 2187–90 doi:101373/clinchem2004040196 PMID 15502097 
  3. ^ Smets EM, Visser A, Go AT, van Vugt JM, Oudejans CB February 2006 "Novel biomarkers in preeclampsia" Clinica Chimica Acta; International Journal of Clinical Chemistry Elsevier BV 364 1-2: 22–32 doi:101016/jcca200506011 PMID 16139262 
  4. ^ Chan KC, Zhang J, Hui AB, Wong N, Lau TK, Leung TN, Lo KW, Huang DW, Lo YM January 2004 "Size distributions of maternal and fetal DNA in maternal plasma" Clinical Chemistry American Association for Clinical Chemistry AACC 50 1: 88–92 doi:101373/clinchem2003024893 PMID 14709639 
  5. ^ Li Y, Zimmermann B, Rusterholz C, Kang A, Holzgreve W, Hahn S June 2004 "Size separation of circulatory DNA in maternal plasma permits ready detection of fetal DNA polymorphisms" PDF Clinical Chemistry American Association for Clinical Chemistry AACC 50 6: 1002–11 doi:101373/clinchem2003029835 PMID 15073090 
  6. ^ Li Y, Di Naro E, Vitucci A, Zimmermann B, Holzgreve W, Hahn S February 2005 "Detection of paternally inherited fetal point mutations for beta-thalassemia using size-fractionated cell-free DNA in maternal plasma" JAMA American Medical Association AMA 293 7: 843–9 doi:101001/jama2937843 PMID 15713774 
  7. ^ Wang E, Batey A, Struble C, Musci T, Song K, Oliphant A July 2013 "Gestational age and maternal weight effects on fetal cell-free DNA in maternal plasma" Prenatal Diagnosis 33 7: 662–6 doi:101002/pd4119 PMID 23553731 
  8. ^ Lo YM, Tein MS, Lau TK, Haines CJ, Leung TN, Poon PM, Wainscoat JS, Johnson PJ, Chang AM, Hjelm NM April 1998 "Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis" American Journal of Human Genetics Elsevier BV 62 4: 768–75 doi:101086/301800 PMID 9529358 
  9. ^ Lo YM, Zhang J, Leung TN, Lau TK, Chang AM, Hjelm NM January 1999 "Rapid clearance of fetal DNA from maternal plasma" American Journal of Human Genetics Elsevier BV 64 1: 218–24 doi:101086/302205 PMID 9915961 
  10. ^ Lo YM, Hjelm NM, Fidler C, Sargent IL, Murphy MF, Chamberlain PF, Poon PM, Redman CW, Wainscoat JS December 1998 "Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma" The New England Journal of Medicine New England Journal of Medicine NEJM/MMS 339 24: 1734–8 doi:101056/nejm199812103392402 PMID 9845707 
  11. ^ Allyse M, Sayres LC, King JS, Norton ME, Cho MK November 2012 "Cell-free fetal DNA testing for fetal aneuploidy and beyond: clinical integration challenges in the US context" Human Reproduction Oxford University Press OUP 27 11: 3123–31 doi:101093/humrep/des286 PMC 3472618  PMID 22863603 
  12. ^ Mujezinovic F, Alfirevic Z September 2007 "Procedure-related complications of amniocentesis and chorionic villous sampling: a systematic review" Obstetrics and Gynecology Ovid Technologies Wolters Kluwer Health 110 3: 687–94 doi:101097/01aog000027882054029e3 PMID 17766619 
  13. ^ a b Lo YM August 2008 "Fetal nucleic acids in maternal plasma" Annals of the New York Academy of Sciences Wiley-Blackwell 1137 1: 140–3 doi:101196/annals1448004 PMID 18837938 
  14. ^ "Reliable Accurate Prenatal non-Invasive Diagnosis" The NHS RAPID project 
  15. ^ a b Hahn S, Chitty LS April 2008 "Noninvasive prenatal diagnosis: current practice and future perspectives" Current Opinion in Obstetrics & Gynecology 20 2: 146–51 doi:101097/GCO0b013e3282f73349 PMID 18388814 
  16. ^ a b Wright CF, Burton H 22 October 2008 "The use of cell-free fetal nucleic acids in maternal blood for non-invasive prenatal diagnosis" Human Reproduction Update Oxford University Press OUP 15 1: 139–51 doi:101093/humupd/dmn047 PMID 18945714 
  17. ^ Lee TJ, Rolnik DL, Menezes MA, McLennan AC, da Silva Costa F April 2018 "Cell-free fetal DNA testing in singleton IVF conceptions" Human Reproduction 33 4: 572–578 doi:101093/humrep/dey033 PMID 29462319 
  18. ^ Dar P, Shani H, Evans MI June 2016 "Cell-free DNA: Comparison of Technologies" Clinics in Laboratory Medicine 36 2: 199–211 doi:101016/jcll201601015 PMID 27235906 
  19. ^ Grace MR, Hardisty E, Dotters-Katz SK, Vora NL, Kuller JA August 2016 "Cell-Free DNA Screening: Complexities and Challenges of Clinical Implementation" Obstetrical & Gynecological Survey 71 8: 477–87 doi:101097/OGX0000000000000342 PMC 5548289  PMID 27526871 
  20. ^ Allen S, Young E, Bowns B April 2017 "Noninvasive prenatal diagnosis for single gene disorders" Current Opinion in Obstetrics & Gynecology 29 2: 73–79 doi:101097/GCO0000000000000347 PMID 28134670 
  21. ^ Guibert J, Benachi A, Grebille AG, Ernault P, Zorn JR, Costa JM August 2003 "Kinetics of SRY gene appearance in maternal serum: detection by real time PCR in early pregnancy after assisted reproductive technique" Human Reproduction 18 8: 1733–6 PMID 12871892 
  22. ^ Chiu RW, Poon LL, Lau TK, Leung TN, Wong EM, Lo YM September 2001 "Effects of blood-processing protocols on fetal and total DNA quantification in maternal plasma" Clinical Chemistry 47 9: 1607–13 PMID 11514393 
  23. ^ Legler TJ, Liu Z, Mavrou A, Finning K, Hromadnikova I, Galbiati S, Meaney C, Hultén MA, Crea F, Olsson ML, Maddocks DG, Huang D, Fisher SA, Sprenger-Haussels M, Soussan AA, van der Schoot CE September 2007 "Workshop report on the extraction of foetal DNA from maternal plasma" Prenatal Diagnosis Wiley-Blackwell 27 9: 824–9 doi:101002/pd1783 PMID 17604339 
  24. ^ Dhallan R, Au WC, Mattagajasingh S, Emche S, Bayliss P, Damewood M, Cronin M, Chou V, Mohr M March 2004 "Methods to increase the percentage of free fetal DNA recovered from the maternal circulation" JAMA American Medical Association AMA 291 9: 1114–9 doi:101001/jama29191114 PMID 14996781 
  25. ^ Benachi A, Yamgnane A, Olivi M, Dumez Y, Gautier E, Costa JM January 2005 "Impact of formaldehyde on the in vitro proportion of fetal DNA in maternal plasma and serum" Clinical Chemistry American Association for Clinical Chemistry AACC 51 1: 242–4 doi:101373/clinchem2004038125 PMID 15514098 
  26. ^ Chinnapapagari SK, Holzgreve W, Lapaire O, Zimmermann B, Hahn S March 2005 "Treatment of maternal blood samples with formaldehyde does not alter the proportion of circulatory fetal nucleic acids DNA and mRNA in maternal plasma" Clinical Chemistry American Association for Clinical Chemistry AACC 51 3: 652–5 doi:101373/clinchem2004042119 PMID 15738521 
  27. ^ a b Traeger-Synodinos J 2006 "Real-time PCR for prenatal and preimplantation genetic diagnosis of monogenic diseases" Molecular Aspects of Medicine Elsevier BV 27 2-3: 176–91 doi:101016/jmam200512004 PMID 16430951 
  28. ^ Boon EM, Schlecht HB, Martin P, Daniels G, Vossen RH, den Dunnen JT, Bakker B, Elles R October 2007 "Y chromosome detection by Real Time PCR and pyrophosphorolysis-activated polymerisation using free fetal DNA isolated from maternal plasma" Prenatal Diagnosis Wiley-Blackwell 27 10: 932–7 doi:101002/pd1804 PMID 17600849 
  29. ^ Hill M, Pařízek A, Cibula D, Kancheva R, Jirásek JE, Jirkovská M, Velíková M, Kubátová J, Klímková M, Pašková A, Zižka Z, Kancheva L, Kazihnitková H, Zamrazilová L, Stárka L October 2010 "Steroid metabolome in fetal and maternal body fluids in human late pregnancy" The Journal of Steroid Biochemistry and Molecular Biology Elsevier BV 122 4: 114–32 doi:101016/jjsbmb201005007 PMID 20580824 
  30. ^ Al-Yatama MK, Mustafa AS, Ali S, Abraham S, Khan Z, Khaja N May 2001 "Detection of Y chromosome-specific DNA in the plasma and urine of pregnant women using nested polymerase chain reaction" Prenatal Diagnosis Wiley-Blackwell 21 5: 399–402 doi:101002/pd69 PMID 11360283 
  31. ^ a b Zimmermann BG, Grill S, Holzgreve W, Zhong XY, Jackson LG, Hahn S December 2008 "Digital PCR: a powerful new tool for noninvasive prenatal diagnosis" Prenatal Diagnosis Wiley-Blackwell 28 12: 1087–93 doi:101002/pd2150 PMID 19003785 
  32. ^ a b Lo YM, Lun FM, Chan KC, Tsui NB, Chong KC, Lau TK, Leung TY, Zee BC, Cantor CR, Chiu RW August 2007 "Digital PCR for the molecular detection of fetal chromosomal aneuploidy" Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences 104 32: 13116–21 doi:101073/pnas0705765104 PMC 1934923  PMID 17664418 
  33. ^ Quake S July 2007 "At the interface of physics and biology" BioTechniques 43 1: 19 PMID 17695250 
  34. ^ Chiu RW, Lo YM November 2010 "Pregnancy-associated microRNAs in maternal plasma: a channel for fetal-maternal communication" Clinical Chemistry American Association for Clinical Chemistry AACC 56 11: 1656–7 doi:101373/clinchem2010153684 PMID 20837782 
  35. ^ a b Ding C 2008 "MALDI-TOF Mass Spectrometry for Analyzing Cell-Free Fetal DNA in Maternal Plasma" Methods in Molecular Biology Totowa, NJ: Humana Press 444: 253–67 doi:101007/978-1-59745-066-9_20 ISBN 978-1-58829-803-4 PMID 18425487 
  36. ^ Akolekar R, Farkas DH, VanAgtmael AL, Bombard AT, Nicolaides KH October 2010 "Fetal sex determination using circulating cell-free fetal DNA ccffDNA at 11 to 13 weeks of gestation" Prenatal Diagnosis Wiley-Blackwell 30 10: 918–23 doi:101002/pd2582 PMID 20721878 
  37. ^ Tong YK, Chiu RW, Chan KC, Leung TY, Lo YM September 2012 "Technical concerns about immunoprecipitation of methylated fetal DNA for noninvasive trisomy 21 diagnosis" Nature Medicine Springer Nature 18 9: 1327–8; author reply 1328–9 doi:101038/nm2915 PMID 22961155 
  38. ^ Papageorgiou EA, Karagrigoriou A, Tsaliki E, Velissariou V, Carter NP, Patsalis PC April 2011 "Fetal-specific DNA methylation ratio permits noninvasive prenatal diagnosis of trisomy 21" Nature Medicine Springer Nature 17 4: 510–3 doi:101038/nm2312 PMC 3977039  PMID 21378977 
  39. ^ a b White HE, Dent CL, Hall VJ, Crolla JA, Chitty LS 14 September 2012 Oudejans C, ed "Evaluation of a novel assay for detection of the fetal marker RASSF1A: facilitating improved diagnostic reliability of noninvasive prenatal diagnosis" PLOS One Public Library of Science PLoS 7 9: e45073 doi:101371/journalpone0045073 PMC 3443218  PMID 23024794 
  40. ^ Ng EK, Tsui NB, Lam NY, Chiu RW, Yu SC, Wong SC, Lo ES, Rainer TH, Johnson PJ, Lo YM August 2002 "Presence of filterable and nonfilterable mRNA in the plasma of cancer patients and healthy individuals" Clinical Chemistry 48 8: 1212–7 PMID 12142376 
  41. ^ Baird PA, Anderson TW, Newcombe HB, Lowry RB May 1988 "Genetic disorders in children and young adults: a population study" American Journal of Human Genetics 42 5: 677–93 PMC 1715177  PMID 3358420 
  42. ^ Scheffer PG, van der Schoot CE, Page-Christiaens GC, Bossers B, van Erp F, de Haas M January 2010 "Reliability of fetal sex determination using maternal plasma" Obstetrics and Gynecology Ovid Technologies Wolters Kluwer Health 115 1: 117–26 doi:101097/aog0b013e3181c3c938 PMID 20027043 
  43. ^ a b Bustamante-Aragones A, Gonzalez-Gonzalez C, de Alba MR, Ainse E, Ramos C March 2010 "Noninvasive prenatal diagnosis using ccffDNA in maternal blood: state of the art" Expert Review of Molecular Diagnostics Informa UK Limited 10 2: 197–205 doi:101586/erm0986 PMID 20214538 
  44. ^ Zimmermann B, El-Sheikhah A, Nicolaides K, Holzgreve W, Hahn S September 2005 "Optimized real-time quantitative PCR measurement of male fetal DNA in maternal plasma" Clinical Chemistry American Association for Clinical Chemistry AACC 51 9: 1598–604 doi:101373/clinchem2005051235 PMID 16020496 
  45. ^ Finning KM, Chitty LS April 2008 "Non-invasive fetal sex determination: impact on clinical practice" Seminars in Fetal & Neonatal Medicine Elsevier BV 13 2: 69–75 doi:101016/jsiny200712007 PMID 18243829 
  46. ^ Markey CM, Wadia PR, Rubin BS, Sonnenschein C, Soto AM June 2005 "Long-term effects of fetal exposure to low doses of the xenoestrogen bisphenol-A in the female mouse genital tract" Biology of Reproduction Oxford University Press OUP 72 6: 1344–51 doi:101095/biolreprod104036301 PMID 15689538 
  47. ^ Sayres LC, Cho MK July 2011 "Cell-free fetal nucleic acid testing: a review of the technology and its applications" Obstetrical & Gynecological Survey Ovid Technologies Wolters Kluwer Health 66 7: 431–42 doi:101097/ogx0b013e31822dfbe2 PMID 21944155 
  48. ^ Hill M, Barrett AN, White H, Chitty LS October 2012 "Uses of cell free fetal DNA in maternal circulation" Best Practice & Research Clinical Obstetrics & Gynaecology Elsevier BV 26 5: 639–54 doi:101016/jbpobgyn201203004 PMID 22542961 
  49. ^ Norbury G, Norbury CJ April 2008 "Non-invasive prenatal diagnosis of single gene disorders: how close are we" Seminars in Fetal & Neonatal Medicine Elsevier BV 13 2: 76–83 doi:101016/jsiny200712008 PMID 18234572 
  50. ^ a b Li Y, Page-Christiaens GC, Gille JJ, Holzgreve W, Hahn S January 2007 "Non-invasive prenatal detection of achondroplasia in size-fractionated cell-free DNA by MALDI-TOF MS assay" Prenatal Diagnosis Wiley-Blackwell 27 1: 11–7 doi:101002/pd1608 PMID 17154237 
  51. ^ de Die-Smulders CE, de Wert GM, Liebaers I, Tibben A, Evers-Kiebooms G 2013 "Reproductive options for prospective parents in families with Huntington's disease: clinical, psychological and ethical reflections" Human Reproduction Update 19 3: 304–15 doi:101093/humupd/dms058 PMID 23377865 
  52. ^ Fan HC, Blumenfeld YJ, Chitkara U, Hudgins L, Quake SR October 2008 "Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood" Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences 105 42: 16266–71 doi:101073/pnas0808319105 PMC 2562413  PMID 18838674 
  53. ^ a b Cardo L, García BP, Alvarez FV August 2010 "Non-invasive fetal RHD genotyping in the first trimester of pregnancy" Clinical Chemistry and Laboratory Medicine Walter de Gruyter GmbH 48 8: 1121–6 doi:101515/cclm2010234 PMID 20482298 
  54. ^ Chinen PA, Nardozza LM, Martinhago CD, Camano L, Daher S, Pares DB, Minett T, Araujo Júnior E, Moron AF November 2010 "Noninvasive determination of fetal rh blood group, D antigen status by cell-free DNA analysis in maternal plasma: experience in a Brazilian population" American Journal of Perinatology Georg Thieme Verlag KG 27 10: 759–62 doi:101055/s-0030-1253560 PMID 20408112 
  55. ^ Okwundu CI, Afolabi BB January 2013 "Intramuscular versus intravenous anti-D for preventing Rhesus alloimmunization during pregnancy" The Cochrane Database of Systematic Reviews 1: CD007885 doi:101002/14651858CD007885pub2 PMID 23440818 
  56. ^ Aykut A, Onay H, Sagol S, Gunduz C, Ozkinay F, Cogulu O December 2013 "Determination of fetal rhesus d status by maternal plasma DNA analysis" Balkan Journal of Medical Genetics Walter de Gruyter GmbH 16 2: 33–8 doi:102478/bjmg-2013-0029 PMC 4001413  PMID 24778561 
  57. ^ Svobodová I, Pazourková E, Hořínek A, Novotná M, Calda P, Korabečná M 2015 "Performance of Droplet Digital PCR in Non-Invasive Fetal RHD Genotyping - Comparison with a Routine Real-Time PCR Based Approach" PLOS One 10 11: e0142572 doi:101371/journalpone0142572 PMC 4642940  PMID 26562517 
  58. ^ Papasavva T, Martin P, Legler TJ, Liasides M, Anastasiou G, Christofides A, Christodoulou T, Demetriou S, Kerimis P, Kontos C, Leontiades G, Papapetrou D, Patroclos T, Phylaktou M, Zottis N, Karitzie E, Pavlou E, Kountouris P, Veldhuisen B, van der Schoot E, Kleanthous M April 2016 "Prevalence of RhD status and clinical application of non-invasive prenatal determination of fetal RHD in maternal plasma: a 5 year experience in Cyprus" BMC Research Notes Springer Nature 9 1: 198 doi:101186/s13104-016-2002-x PMC 4818414  PMID 27036548 
  59. ^ Zhang B, Lu BY, Yu B, Zheng FX, Zhou Q, Chen YP, Zhang XQ April 2017 "Noninvasive prenatal screening for fetal common sex chromosome aneuploidies from maternal blood" The Journal of International Medical Research SAGE Publications 45 2: 621–630 doi:101177/0300060517695008 PMC 5536640  PMID 28357876 
  60. ^ a b c Kazemi M, Salehi M, Kheirollahi M 10 August 2016 "Down Syndrome: Current Status, Challenges and Future Perspectives" International Journal of Molecular and Cellular Medicine 5 3: 125–133 PMC 5125364  PMID 27942498 
  61. ^ Mersy E, Smits LJ, van Winden LA, de Die-Smulders CE, Paulussen AD, Macville MV, Coumans AB, Frints SG 2013 "Noninvasive detection of fetal trisomy 21: systematic review and report of quality and outcomes of diagnostic accuracy studies performed between 1997 and 2012" Human Reproduction Update 19 4: 318–29 doi:101093/humupd/dmt001 PMID 23396607 
  62. ^ Clark-Ganheart CA, Iqbal SN, Brown DL, Black S, Fries MH May 2014 "Understanding the Limitations of Circulating Cell Free Fetal DNA: An Example of Two Unique Cases" Journal of Clinical Gynecology and Obstetrics 3 2: 38–70 doi:1014740/jcgo229w PMC 4185925  PMID 25298847 
  63. ^ Wataganara T, LeShane ES, Farina A, Messerlian GM, Lee T, Canick JA, Bianchi DW February 2003 "Maternal serum cell-free fetal DNA levels are increased in cases of trisomy 13 but not trisomy 18" Human Genetics 112 2: 204–8 doi:101007/s00439-002-0853-9 inactive 2018-05-12 PMID 12522563 
  64. ^ Chiu RW, Lo YM April 2011 "Non-invasive prenatal diagnosis by fetal nucleic acid analysis in maternal plasma: the coming of age" Seminars in Fetal & Neonatal Medicine Elsevier BV 16 2: 88–93 doi:101016/jsiny201010003 PMID 21075065 
  65. ^ Noninvasive Prenatal Diagnosis of Fetal Aneuploidy Using Cell-Free Fetal Nucleic Acids in Maternal Blood: Clinical Policy Effective 05/01/2013 from Oxford Health Plans
  66. ^ Bianchi DW, Parker RL, Wentworth J, Madankumar R, Saffer C, Das AF, Craig JA, Chudova DI, Devers PL, Jones KW, Oliver K, Rava RP, Sehnert AJ February 2014 "DNA sequencing versus standard prenatal aneuploidy screening" The New England Journal of Medicine 370 9: 799–808 doi:101056/NEJMoa1311037 PMID 24571752  A recent study in the New England Journal of Medicine demonstrated the feasibility of using NIPT in a low risk population
  67. ^ Henderson JT, Thompson JH, Burda BU, Cantor A April 2017 "Preeclampsia Screening: Evidence Report and Systematic Review for the US Preventive Services Task Force" JAMA American Medical Association AMA 317 16: 1668–1683 doi:101001/jama201618315 PMID 28444285 
  68. ^ a b Seval MM, Karabulut HG, Tükün A, Koç A 2015 "Cell free fetal DNA in the plasma of pregnant women with preeclampsia" Clinical and Experimental Obstetrics & Gynecology 42 6: 787–91 PMID 26753487 
  69. ^ Lo YM, Lau TK, Zhang J, Leung TN, Chang AM, Hjelm NM, Elmes RS, Bianchi DW October 1999 "Increased fetal DNA concentrations in the plasma of pregnant women carrying fetuses with trisomy 21" Clinical Chemistry 45 10: 1747–51 PMID 10508120 
  70. ^ Leung TN, Zhang J, Lau TK, Chan LY, Lo YM January 2001 "Increased maternal plasma fetal DNA concentrations in women who eventually develop preeclampsia" Clinical Chemistry 47 1: 137–9 PMID 11148193 
  71. ^ Zhong XY, Holzgreve W, Hahn S 2002 "The levels of circulatory cell free fetal DNA in maternal plasma are elevated prior to the onset of preeclampsia" Hypertension in Pregnancy Informa UK Limited 21 1: 77–83 doi:101081/prg-120002911 PMID 12044339 
  72. ^ Yurkiewicz IR, Korf BR, Lehmann LS January 2014 "Prenatal whole-genome sequencing--is the quest to know a fetus's future ethical" The New England Journal of Medicine 370 3: 195–7 doi:101056/NEJMp1215536 PMID 24428465 
  73. ^ Wellcome Trust Case Control Consortium June 2007 "Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls" Nature 447 7145: 661–78 doi:101038/nature05911 PMC 2719288  PMID 17554300 
  74. ^ Mailman MD, Feolo M, Jin Y, Kimura M, Tryka K, Bagoutdinov R, et al October 2007 "The NCBI dbGaP database of genotypes and phenotypes" Nature Genetics 39 10: 1181–6 doi:101038/ng1007-1181 PMC 2031016  PMID 17898773 

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